Yuan Jianqin, Chang Hong, Zhao Jianghe, Shi Zongyong, Wang Jundong
College of Life Science, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2016 May 25;32(5):657-668. doi: 10.13345/j.cjb.150530.
Metabolism and deposition of exogenous gene and protein from transgenic glyphosate herbicide-tolerant soybean meal in SD rats were studied in the experiment. The transgenic soybean GTS40-3-2 meal and its non-transgenic counterpart (parent A5403) were fed to the generation and the second generation Sprague-Dawley (SD) rats (Rattus norvegicus). The study added the genetically modified (GM) soybean meal and its non-transgenic control soybean meal (parent A5403) in a ratio of 20% respectively to the feeds. By using qualitative, quantitative PCR and ELISA methods to detect transgenic soybean residues of metabolism ingredients in rats, the safety and influence of GM soybean were evaluated. The results showed that the intestinal fecal and cecum contents of rats were detected with residues of GM ingredients, intestinal flora and organs were not found related genes and protein. These results indicated that transgenic glyphosate herbicide-tolerant soybean GTS40-3-2 meal was as safe as its non-GM soybean meal in long-term feeding study.
本实验研究了转基因抗草甘膦大豆粕中外源基因和蛋白质在SD大鼠体内的代谢及沉积情况。将转基因大豆GTS40-3-2粕及其非转基因对照（亲本A5403）分别喂给第一代和第二代斯普拉格-道利（SD）大鼠（褐家鼠）。该研究以20%的比例分别向饲料中添加转基因大豆粕及其非转基因对照大豆粕（亲本A5403）。通过定性、定量PCR和ELISA方法检测大鼠体内转基因大豆代谢成分的残留，评估转基因大豆的安全性和影响。结果显示，在大鼠的肠道粪便和盲肠内容物中检测到了转基因成分残留，在肠道菌群和器官中未发现相关基因和蛋白质。这些结果表明，在长期喂养研究中，转基因抗草甘膦大豆GTS40-3-2粕与其非转基因大豆粕一样安全。
