Hassanzarei Shekoufeh, Hashemi Mohammad, Sattarifard Hedieh, Hashemi Seyed Mehdi, Bahari Gholamreza, Ghavami Saeid
1 Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
2 Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Tumour Biol. 2017 Oct;39(10):1010428317727539. doi: 10.1177/1010428317727539.
There is an increasing body of evidence which highlights the critical functions of long non-coding RNAs in the carcinogenicity mechanism of a variety of cancers. It has been reported that HOX transcript antisense intergenic RNA, a member of long non-coding RNA family, increases breast cancer risk. To date, no data regarding the association between HOX transcript antisense intergenic RNA polymorphisms and the risk of breast cancer development has been reported in Iran. Here, we examine the possible association between HOX transcript antisense intergenic RNA gene polymorphisms and breast cancer in a sample of southeast Iranian female population. The HOX transcript antisense intergenic RNA rs920778, rs12826786, rs4759314, and 1899663 gene polymorphisms were genotyped in 220 cases and 231 controls by polymerase chain reaction-restriction fragment length polymorphism. Our findings indicated that rs920778 polymorphism has significant positive association with breast cancer; rs12826786 and rs1899663 polymorphisms demonstrated significant negative association with breast cancer; and the rs4759314 variant was not associated with breast cancer risk. Haplotype analysis revealed that TGAC, CTAT, and TTAT haplotypes significantly decreased the risk of breast cancer compared with rs920778T/rs1899663G/rs4759314A/rs12826786T haplotype. In conclusion, we investigated only four variants of HOX transcript antisense intergenic RNA gene, and the findings suggest that HOX transcript antisense intergenic RNA rs920778, rs12826786, and rs1899663 polymorphisms may be associated with breast cancer risk in a sample of southeast Iranian population. Further replication studies with other polymorphisms of HOX transcript antisense intergenic RNA gene involving a greater sample size and different ethnicities are necessary to verify our findings.
越来越多的证据表明，长链非编码RNA在多种癌症的致癌机制中发挥着关键作用。据报道，长链非编码RNA家族成员HOX转录反义基因间RNA会增加患乳腺癌的风险。迄今为止，伊朗尚未有关于HOX转录反义基因间RNA多态性与乳腺癌发生风险之间关联的数据报道。在此，我们在伊朗东南部女性人群样本中研究HOX转录反义基因间RNA基因多态性与乳腺癌之间的可能关联。通过聚合酶链反应-限制性片段长度多态性方法，对220例病例和231例对照进行HOX转录反义基因间RNA rs920778、rs12826786、rs4759314和1899663基因多态性基因分型。我们的研究结果表明，rs920778多态性与乳腺癌存在显著正相关；rs12826786和rs1899663多态性与乳腺癌呈显著负相关；而rs4759314变异与乳腺癌风险无关。单倍型分析显示，与rs920778T/rs1899663G/rs4759314A/rs12826786T单倍型相比，TGAC、CTAT和TTAT单倍型显著降低了患乳腺癌的风险。总之，我们仅研究了HOX转录反义基因间RNA基因的四个变异体，研究结果表明，在伊朗东南部人群样本中，HOX转录反义基因间RNA rs920778、rs12826786和rs1899663多态性可能与乳腺癌风险相关。有必要进行进一步的重复研究，涉及HOX转录反义基因间RNA基因的其他多态性，样本量更大且种族不同，以验证我们的研究结果。
