Human Nutrition Unit, Faculty of Medicine and Health Sciences, Institute of Health Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
Mol Nutr Food Res. 2018 Jan;62(2). doi: 10.1002/mnfr.201700594. Epub 2017 Dec 11.
To analyze the effect of three calorie-restricted diets with different amount and quality of carbohydrates on subcutaneous adipose tissue (SAT) microRNA (miRNA) profile.
6-month parallel, randomized trial conducted on overweight and obese subjects randomized to: 1) low glycemic index diet (LGI), 2) high glycemic index diet (HGI), and 3) low-fat (LF). The genome-wide SAT miRNA profile was assessed in eight randomly selected participants and the most relevant changing miRNAs (n = 13) were validated in 48 subjects. None of the miRNAs showed significant changes between the intervention groups. However, changes in some of them correlated with changes in biochemical and anthropometric variables. Stratifying our population according to tertiles of percentage change in body weight (BW), we observed a significant down-regulation of miR-210 in those subjects in Tertile 1 as compared to Tertile 3. When our population was stratified by tertiles of waist circumference, miR-132, miR-29a, miR-34a, and miR-378 were found to be significantly down-regulated, in T2 compared to T3. Furthermore, when stratified by tertiles of fat mass, we also observed the significant down-regulation of miR-132 in T1.
The macronutrient composition of a calorie-restricted diet does not affect the expression of the miRNAs analyzed, while changes in adiposity play a primary regulatory role.
分析三种不同热量和碳水化合物质量限制饮食对皮下脂肪组织 (SAT) 微小 RNA (miRNA) 谱的影响。
在超重和肥胖受试者中进行了为期 6 个月的平行、随机试验，将其随机分为 1) 低升糖指数饮食（LGI）、2) 高升糖指数饮食（HGI）和 3) 低脂肪（LF）组。对 8 名随机选择的参与者的 SAT 全基因组 miRNA 谱进行了评估，并在 48 名参与者中验证了最相关的变化 miRNA（n=13）。干预组之间没有发现 miRNA 有显著变化。然而，其中一些 miRNA 的变化与生化和人体测量变量的变化相关。根据体重变化百分比的三分位数（BW）对我们的人群进行分层，我们观察到 Tertile1 组的 miR-210 与 Tertile3 组相比明显下调。当我们根据腰围的三分位数对人群进行分层时，发现 miR-132、miR-29a、miR-34a 和 miR-378 在 T2 组与 T3 组相比明显下调。此外，当按脂肪量的三分位数分层时，我们还观察到 T1 组的 miR-132 明显下调。
热量限制饮食的宏量营养素组成不会影响分析的 miRNA 的表达，而脂肪量的变化则起着主要的调节作用。
