Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine and the Biomedical Engineering Research Center, Asan Institute of Life Sciences 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea.
Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University College of Medicine 59 Daesangwan-ro, Yongsan-gu, Seoul, Republic of Korea.
J Biophotonics. 2018 Apr;11(4):e201700167. doi: 10.1002/jbio.201700167. Epub 2018 Jan 29.
Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio-optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio-optical sensor, that transduces the presence of the target DNA based on binding-induced changes in the refractive index on the waveguide surface in a label-free and real-time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20 min), 1-step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin.
最近的人畜共患病暴发，如寨卡病毒、中东呼吸综合征和埃博拉病毒，突显了需要快速和准确的诊断检测方法，以帮助控制病原体。Q 热是一种由贝氏柯克斯体传播引起的人畜共患病，可通过气溶胶导致人类严重疾病，被认为是一种潜在的生物恐怖主义剂。然而，由于实际样本中该病原体的水平较低，现有的检测方法并不适合检测这种病原体。我们在这里描述了一种用于准确检测 Q 热的快速生物光学传感器，并验证了其临床实用性。通过将生物光学传感器与等温 DNA 扩增相结合，该传感器以无标记和实时的方式根据结合诱导的波导表面折射率变化来转导目标 DNA 的存在，这种新的诊断工具提供了一种快速(<20 分钟)、1 步 DNA 扩增/检测方法。我们通过检测 11 份来自急性 Q 热肝炎患者的福尔马林固定、石蜡包埋的肝活检样本和 16 份因 Q 热导致不明原因发热的患者的血浆样本中的 C. burnetii，证实了生物光学传感器的临床灵敏度(>90%)。
