Farrokhi Molood, Moallemi Samaneh, Shirkoohi Reza, Golmohammadi Reza, Ahsani-Nasab Sara, Sardashti Sara, Abbasian Ladan, Baesi Kazem, Mohraz Minoo
Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran.
Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2017 Sep;46(9):1256-1264.
This study aimed to determine drug resistance mutations in patients with virological failure and find correlation between HIV drug resistance test and viral load.
Blood sample was collected from 51 patients who suspicious treatment failure in the center of Imam Khomeini Hospital, Tehran, Iran in 2015. Viral voluntary counseling and testing load test was done and the patients with viral load above 1000 copies choose for detection of drug resistance mutations by genotyping method (29 patients).
The majority of patients (82.75) harbored the HIV subtype CRF 35 A-D. The 86.2% patients compromised at least one resistance mutation. The analysis of reverse transcriptase showed M184V (68.9%), T215YISF (44.8%), K103N (27.6%) and the analysis results of protease revealed G73SC (13.8%) and I47VA (6.9%). Eventually, the significant correlation between viral load and drug resistance was found.
The result of our research stress the significance of recognizing drug resistant on time that prohibits the accumulation of drug resistance mutation and circulates the resistance strain of HIV-1 virus and the importance of national study according to the reliable findings for treatment guidelines.
本研究旨在确定病毒学失败患者的耐药性突变，并找出HIV耐药性检测与病毒载量之间的相关性。
2015年从伊朗德黑兰伊玛目霍梅尼医院中心51例疑似治疗失败的患者中采集血样。进行了病毒自愿咨询和检测载量测试，选择病毒载量高于1000拷贝的患者通过基因分型方法检测耐药性突变（29例患者）。
大多数患者（82.75%）携带HIV CRF 35 A-D亚型。86.2%的患者至少存在一种耐药性突变。逆转录酶分析显示M184V（68.9%）、T215YISF（44.8%）、K103N（27.6%），蛋白酶分析结果显示G73SC（13.8%）和I47VA（6.9%）。最终，发现病毒载量与耐药性之间存在显著相关性。
我们的研究结果强调了及时识别耐药性的重要性，这可以防止耐药性突变的积累，传播HIV-1病毒的耐药菌株，以及根据可靠研究结果制定国家治疗指南的重要性。
