Wang Yulin, Li Hui, Wang Xiaohui, Gao Fang, Yu Lan, Chen Xiufeng
Department of Hepatopancreatobiliary and Splenic Medicine, Affiliated Hospital, Logistics University of People's Armed Policed ForceTianjin, China.
Department of Gastroenterology, Navy General Hospital of Chinese PLABeijing, China.
Int J Mol Epidemiol Genet. 2017 Sep 1;8(4):45-52. eCollection 2017.
Gastric cancer (GC) is the 5th most prevalent cancer. The etiology of GC is still poorly understood. We performed a case-control study in a Chinese population to investigate the association of rs2243248 (-1098 G/T), rs2227284 (-33 C/T), rs2243250 (-589 T/C) and rs2070874 (-107 T/C) polymorphisms and haplotypes with the development of gastric cancer in a Chinese population. A total of 362 patients with gastric cancer and 384 controls were recruited between December 2013 and December 2015. Genotyping of rs2243248 (-1098 G/T), rs2227284 (-33 C/T), rs2243250 (-589 T/C) and rs2070874 (-107 T/C) was performed in a 384-well plate format on the sequenom MassARRAY platform, and analyzed by MALDI-TOF MS. The TC and CC genotypes of rs2243250 (-589 T/C) were associated with an increased risk of gastric cancer when compared with the TT genotype, with adjusted ORs (95% CI) of 1.52 (1.07-2.15) and 2.13 (1.30-3.51), respectively. The TTTT haplotype revealed a reduced risk of gastric cancer (OR=0.65, 95% CI=0.45-0.94). No linkage disequilibrium was found among IL-4 rs2243248, rs2227284, rs2243250 and rs2070874. In summary, our findings support a significant association of IL-4 rs2243250 polymorphism with the risk of gastric cancer in the Chinese population, and IL-4 haplotype contributes to the development of this disease.
胃癌（GC）是第五大常见癌症。目前对胃癌的病因仍知之甚少。我们在中国人群中开展了一项病例对照研究，以调查rs2243248（-1098 G/T）、rs2227284（-33 C/T）、rs2243250（-589 T/C）和rs2070874（-107 T/C）基因多态性及单倍型与中国人群胃癌发生的关联。2013年12月至2015年12月期间，共招募了362例胃癌患者和384名对照。在Sequenom MassARRAY平台上以384孔板形式对rs2243248（-1098 G/T）、rs2227284（-33 C/T）、rs2243250（-589 T/C）和rs2070874（-107 T/C）进行基因分型，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行分析。与TT基因型相比，rs2243250（-589 T/C）的TC和CC基因型与胃癌风险增加相关，校正后的比值比（95%可信区间）分别为1.52（1.07-2.15）和2.13（1.30-3.51）。TTTT单倍型显示胃癌风险降低（比值比=0.65，95%可信区间=0.45-0.94）。在白细胞介素-4的rs2243248、rs2227284、rs2243250和rs2070874之间未发现连锁不平衡。总之，我们的研究结果支持白细胞介素-4 rs2243250基因多态性与中国人群胃癌风险显著相关，且白细胞介素-4单倍型促成了该疾病的发生。
