邻苯二甲酸二（2-乙基己基）酯（DEHP）对体外培养大鼠卵巢颗粒细胞增殖及甾体激素合成的影响。
Effect of mono-(2-ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro.
机构信息
Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, Jilin, China.
Department of Disciplines and Research Management, The Second Hospital Affiliated to Dalian Medical University, Dalian, Liaoning, China.
出版信息
J Cell Physiol. 2018 Apr;233(4):3629-3637. doi: 10.1002/jcp.26224. Epub 2017 Nov 24.
This study aimed to examine the proliferation of and secretion by rat ovarian granulosa cells (GCs) treated with mono-(2-ethylhexyl) phthalate (MEHP). Ovarian GCs were incubated with MEHP at concentration of 0, 25, 50, 100, and 200 µM for 24 hr. Cell viability was determined using the MTT Cell Proliferation Assay. Progesterone and estradiol production was evaluated by radioimmunoassay (RIA) and the expression of FSHR, PR, and ER was measured by immunocytochemistry. StAR, P450scc, 3β-HSD, 17β-HSD, and P arom mRNA levels were determined by RT-PCR. MEHP markedly attenuated proliferation of GCs, increased expression of sex hormone receptors and key enzymes in progesterone production, and stimulated steroid hormone secretion. The result of these analyses demonstrates that MEHP exposure of GCs may have effects on rat ovarian functions.
本研究旨在探讨邻苯二甲酸单（2-乙基己基）酯（MEHP）处理后大鼠卵巢颗粒细胞（GCs）的增殖和分泌情况。将卵巢 GCs 用浓度为 0、25、50、100 和 200 μM 的 MEHP 孵育 24 小时。使用 MTT 细胞增殖测定法测定细胞活力。通过放射免疫分析（RIA）评估孕酮和雌二醇的产生，并用免疫细胞化学测定法测量 FSHR、PR 和 ER 的表达。通过 RT-PCR 测定 StAR、P450scc、3β-HSD、17β-HSD 和 P arom mRNA 水平。MEHP 明显抑制 GCs 的增殖，增加孕激素产生中的性激素受体和关键酶的表达，并刺激甾体激素的分泌。这些分析的结果表明，MEHP 暴露于 GCs 可能对大鼠卵巢功能产生影响。
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