Deng Wenwen, Quan Yuan, Yang Shengzhi, Guo Lijuan, Zhang Xiuli, Liu Shuliang, Chen Shujuan, Zhou Kang, He Li, Li Bei, Gu Yunfu, Zhao Shaohua, Zou Likou
1 Department of Applied Microbiology, College of Resources, Sichuan Agricultural University , Chengdu, People's Republic of China .
2 Clinical Laboratory, Taizhou Second People's Hospital , Jiangyan, People's Republic of China .
Microb Drug Resist. 2018 Jul/Aug;24(6):782-791. doi: 10.1089/mdr.2017.0127. Epub 2017 Oct 17.
This study aims to demonstrate the antibiotic resistance and its association with disinfectant and heavy metal resistance in 152 Salmonella isolates recovered from retail foods of animal origins. Susceptibility testing demonstrated that 92.8% isolates were resistant to at least one antibiotic, and the resistance was highest to oxytetracycline (80.9%), followed by trimethoprim (64.5%), amoxicillin (28.9%), ampicillin (28.3%), levofloxacin (21.7%), ciprofloxacin (16.4%), and gentamicin (10.5%), respectively. The bla and tetA genes (44.7%) were commonly present. The qacF and qacEΔ1 genes were detected in 18.4% and 8.6% of all isolates. The Cu-resistance genes pcoR, pcoC, and pcoA were the most prevalent (20.4-40.8%), followed by Hg-resistance gene merA (17.8%) and As-resistance genes arsB (6.6%). The antibiotic resistance was highly associated with disinfectant or certain heavy metal resistance genes. Most notably, the association among Cu-resistance genes (pcoC, pcoR), disinfectant resistance genes (qacF, qacEΔ1), and tetracycline and sulfonamide resistance genes (tet, sul) was significant (p < 0.05). Pulsed-field gel electrophoresis revealed that Salmonella isolates was associated with supermarkets indicating the possibility of crosscontamination in farms or processing environment. This study indicated that retail meats may be a reservoir for the dissemination of antibiotic-resistant Salmonella and using disinfectants for decontamination or metals in livestock may provide a pressure for coselecting strains with acquired resistance to other antimicrobials.
本研究旨在阐明从动物源性零售食品中分离出的152株沙门氏菌的抗生素耐药性及其与消毒剂和重金属耐药性的关联。药敏试验表明，92.8%的分离株对至少一种抗生素耐药，其中对土霉素的耐药率最高（80.9%），其次是甲氧苄啶（64.5%）、阿莫西林（28.9%）、氨苄西林（28.3%）、左氧氟沙星（21.7%）、环丙沙星（16.4%）和庆大霉素（10.5%）。bla和tetA基因普遍存在（44.7%）。在所有分离株中，qacF和qacEΔ1基因的检出率分别为18.4%和8.6%。铜耐药基因pcoR、pcoC和pcoA最为常见（20.4 - 40.8%），其次是汞耐药基因merA（17.8%）和砷耐药基因arsB（6.6%）。抗生素耐药性与消毒剂或某些重金属耐药基因高度相关。最显著的是，铜耐药基因（pcoC、pcoR）、消毒剂耐药基因（qacF、qacEΔ1）与四环素和磺胺类耐药基因（tet、sul）之间的关联具有显著性（p < 0.05）。脉冲场凝胶电泳显示，沙门氏菌分离株与超市有关，这表明在养殖场或加工环境中可能存在交叉污染。本研究表明，零售肉类可能是抗生素耐药性沙门氏菌传播的储存库，在牲畜中使用消毒剂进行去污或使用金属可能会为共选择获得对其他抗菌药物耐药性的菌株提供压力。
