State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing 100083, PR China.
Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Biosens Bioelectron. 2018 Mar 15;101:60-65. doi: 10.1016/j.bios.2017.10.008. Epub 2017 Oct 7.
A novel three-dimensional (3D) carbon composite of PANI@CNTs with rhizobium-like structure is prepared by in-situ polymerization of aniline monomers around and along the functionalized carbon nanotubes (CNTs) and then carbonized at 1600°C for enzymatic biofuel cells (EBFCs). The SEM and TEM images clearly show that the carbonized PANI grew seamlessly on the surface of CNTs and presented the rhizobium-like structure. The carbonized PANI acts like conductive "glue" and connects the adjacent tubes together, which can assemble the CNTs into a 3D network. The PANI@CNTs composite modified glassy carbon electrodes based on glucose oxidase (GOx) and laccase (Lac) exhibit high electrochemical performance. A glucose//O EBFC constitutes of the fabricated anode and cathode performs a maximum power density of 1.12mWcm at 0.45V. Furthermore, three of the fabricated EBFCs in series are able to lightening up a yellow light-emitting diode (LED) whose turn-on voltage is about at 1.8V. This work may be helpful for exploiting novel substrates by carbonizing the composites of conducting polymer with nano materials at high-temperature for immobilization of enzymes in the EBFCs or biosensor fields.
一种新型的具有根瘤菌状结构的 PANI@CNTs 三维（3D）碳复合材料，是通过苯胺单体在功能化碳纳米管（CNTs）周围和沿其原位聚合，并在 1600°C 碳化制备得到的，用于酶生物燃料电池（EBFC）。SEM 和 TEM 图像清楚地表明，碳化的 PANI 无缝地生长在 CNTs 的表面上，并呈现出根瘤菌状结构。碳化的 PANI 充当导电“胶水”，将相邻的管连接在一起，将 CNTs 组装成 3D 网络。基于葡萄糖氧化酶（GOx）和漆酶（Lac）的 PANI@CNTs 复合改性玻碳电极具有高电化学性能。由制备的阳极和阴极组成的葡萄糖/O EBFC 在 0.45V 时表现出 1.12mWcm 的最大功率密度。此外，串联的三个制备的 EBFC 能够点亮一个黄色发光二极管（LED），其导通电压约为 1.8V。这项工作可能有助于通过在高温下碳化导电聚合物与纳米材料的复合材料来开发新型基底，用于 EBFC 或生物传感器领域中酶的固定化。
