Department of Food Science and Technology, Institute of Food Science, BOKU - University of Natural Resources and Life Sciences, Vienna, Austria.
Department of Agrobiotechnology, Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology, BOKU - University of Natural Resources and Life Sciences, Vienna, Austria.
Poult Sci. 2017 Sep 1;96(11):4053-4060. doi: 10.3382/ps/pex232.
Increasing antibiotic resistance is a major public health concern. Fluoroquinolones are used to treat and prevent poultry diseases worldwide. Fluoroquinolone resistance rates are high in their countries of use. The aim of this study was to evaluate the effect of an acids-based feed additive, as well as fluoroquinolone antibiotics, on the prevalence of antibiotic-resistant E. coli. A total of 480 broiler chickens (Ross 308) were randomly assigned to 3 treatments: a control group receiving a basal diet; a group receiving a feed additive (FA) based on formic acid, acetic acid and propionic acid; and an antibiotic enrofloxacin (AB) group given the same diet, but supplemented with enrofloxacin in water. A pooled fecal sample of one-day-old chicks was collected upon arrival at the experimental farm. On d 17 and d 38 of the trial, cecal samples from each of the 8 pens were taken, and the count of E. coli and antibiotic-resistant E. coli was determined.The results of the present study show a high prevalence of antibiotic-resistant E. coli in one-day-old chicks. Supplementation of the diet with FA and treatment of broilers with AB did not have a significant influence on the total number of E. coli in the cecal content on d 17 and d 38 of the trial. Supplementation with FA contributed to better growth performance and to a significant decrease (P ≤ 0.05) in E. coli resistant to ampicillin and tetracycline compared to the control and AB groups, as well as to a decrease (P ≤ 0.05) in sulfamethoxazole and ciprofloxacin-resistant E. coli compared to the AB group. Treatment with AB increased (P ≤ 0.05) the average daily weight compared to the control group and increased (P ≤ 0.05) the number of E. coli resistant to ciprofloxacin, streptomycin, sulfamethoxazole and tetracycline; it also decreased (P ≤ 0.05) the number of E. coli resistant to cefotaxime and extended spectrum beta-lactamase- (ESBL-) producing E. coli in the ceca of broilers.
抗生素耐药性的增加是一个主要的公共卫生关注点。氟喹诺酮类药物被广泛用于治疗和预防世界各地的家禽疾病。这些药物在其使用国家的耐药率很高。本研究旨在评估一种基于酸的饲料添加剂以及氟喹诺酮类抗生素对耐药大肠杆菌流行率的影响。将 480 只肉鸡（罗斯 308）随机分为 3 个处理组：对照组接受基础日粮；一组接受基于甲酸、乙酸和丙酸的饲料添加剂（FA）；抗生素恩诺沙星（AB）组给予相同的日粮，但在水中补充恩诺沙星。在实验农场到达时，收集了 1 日龄雏鸡的粪便混合样。在试验的第 17 天和第 38 天，从每个 8 个鸡笼中取出盲肠样本，并确定大肠杆菌和耐药大肠杆菌的数量。本研究结果表明，1 日龄雏鸡中存在高比例的耐药大肠杆菌。日粮中添加 FA 和用 AB 治疗肉鸡对试验第 17 天和第 38 天盲肠内容物中大肠杆菌总数没有显著影响。与对照组和 AB 组相比，FA 的添加有助于改善生长性能，并显著降低（P ≤ 0.05）对氨苄西林和四环素耐药的大肠杆菌数量，以及与 AB 组相比，降低（P ≤ 0.05）对磺胺甲恶唑和环丙沙星耐药的大肠杆菌数量。AB 治疗组与对照组相比，平均日增重增加（P ≤ 0.05），对环丙沙星、链霉素、磺胺甲恶唑和四环素耐药的大肠杆菌数量增加（P ≤ 0.05），对头孢噻肟和产超广谱β-内酰胺酶（ESBL）大肠杆菌的数量减少（P ≤ 0.05）。
