Sangsriratanakul Natthanan, Toyofuku Chiharu, Suzuki Mayuko, Komura Miyuki, Yamada Masashi, Alam Md Shahin, Ruenphet Sakchai, Shoham Dany, Sakai Kouji, Takehara Kazuaki
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan; The United Graduate School of Veterinary Science, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan.
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
J Virol Methods. 2018 Jan;251:83-87. doi: 10.1016/j.jviromet.2017.10.017. Epub 2017 Oct 17.
An alkaline agent, namely, food additive grade calcium hydroxide (FdCa(OH)) in the solution, powder and suspension forms was evaluated as a virucidal agent, using a murine norovirus (MNV) as the surrogate for human norovirus. The main constituent of FdCa(OH) is Ca(OH), which has pH 13 in 0.17% solution. The results showed that 0.17% FdCa(OH) solution could inactivate MNV within 30s even in the presence of organic materials (5% fetal bovine serum (FBS)). In a contaminated surface experiment, MNV with 5% FBS was inoculated on rayon sheets, and the result showed FdCa(OH) solution could markedly reduce virus titer within 1min. When mouse feces were spiked with MNV and FdCa(OH) powder as 10% and 20% w/w was added to the feces, these concentrations could inactivate the virus within 30min and 15min, respectively. Whereas, FdCa(OH) suspension at 2.5% and 5% could inactivate the virus within 30min and at 1% within 45min. These and additional results obtained here indicate that FdCa(OH) is an effective virucidal agent against MNV, and can serve as a useful alternative disinfectant for inactivation and prevention of human norovirus in house and hospital.
使用鼠诺如病毒（MNV）作为人类诺如病毒的替代物，对一种碱性试剂，即溶液、粉末和悬浮液形式的食品添加剂级氢氧化钙（FdCa(OH)）作为杀病毒剂进行了评估。FdCa(OH)的主要成分是Ca(OH)，其在0.17%溶液中的pH值为13。结果表明，即使存在有机物质（5%胎牛血清（FBS）），0.17%的FdCa(OH)溶液也能在30秒内灭活MNV。在污染表面实验中，将含有5%FBS的MNV接种在人造纤维片上，结果表明FdCa(OH)溶液能在1分钟内显著降低病毒滴度。当在小鼠粪便中加入MNV，并分别以10%和20%（w/w）的比例向粪便中添加FdCa(OH)粉末时，这些浓度分别能在30分钟和15分钟内灭活病毒。而2.5%和5%的FdCa(OH)悬浮液能在30分钟内灭活病毒，1%的悬浮液能在45分钟内灭活病毒。此处获得的这些及其他结果表明，FdCa(OH)是一种针对MNV的有效杀病毒剂，可作为家庭和医院中灭活和预防人类诺如病毒的有用替代消毒剂。
