Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Redox Biol. 2018 Apr;14:386-390. doi: 10.1016/j.redox.2017.10.003. Epub 2017 Oct 7.
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25°C during development and shifted to 15°C in adulthood exhibited an even longer lifespan than animals constantly kept at 15°C. Analysis of the in vivo redox status demonstrated that at 25°C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15°C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25°C to 15°C and reveal a lifespan similar to prdx-2 worms always kept at 15°C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms.
在变温动物(如秀丽隐杆线虫)中,仅仅通过降低生长温度就可以显著延长其寿命。为了深入了解这种效应的机制基础,我们研究了温度在发育和成年期对秀丽隐杆线虫寿命的影响。我们发现,在发育过程中暴露于 25°C 并在成年后转移到 15°C 的线虫比始终保持在 15°C 的动物寿命更长。体内氧化还原状态的分析表明,在 25°C 下,秀丽隐杆线虫幼虫的氧化还原状态更还原,Prdx-2 表达水平更高,而在 15°C 下培养的动物则不然。缺乏 prdx-2 的线虫在从 25°C 转移到 15°C 时不会表现出额外的寿命延长,并且其寿命与始终保持在 15°C 的 prdx-2 线虫相似。这些结果表明,在发育过程中短暂改变体内氧化还原状态可能对生物体产生非常有益的长期影响。