Trybus Wojciech, Król Teodora, Trybus Ewa, Kopacz-Bednarska Anna, Król Grzegorz, Karpowicz Ewa
Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland
Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Kielce, Poland.
Anticancer Res. 2017 Nov;37(11):6087-6096. doi: 10.21873/anticanres.12057.
An example of plant-derived compounds that may be of great importance in oncological therapy is emodin. The aim of this study was to evaluate the range of changes in the lysosomal compartment of HeLa cervical cancer cells treated with emodin.
Changes in the lysosomal compartment were evaluated using microscopic techniques. In order to evaluate the activity of lysosomal enzymes and the permeability of the lysosomal membrane, a spectrophotometric technique was employed.
Emodin induced significant changes in the lysosomal compartment, manifesting as an increase in the number of lysosomes, autophagic vacuoles and the activity of lysosomal hydrolases. Emodin exhibited cytotoxic activity against lysosomes through lysosomal membrane damage with possible leaks of lysosomal hydrolases into the cytoplasm.
Emodin induces degradation processes and promotes the death of tumor cells through a mechanism that occurs with clear involvement of the lysosomal compartment.
大黄素是一种可能在肿瘤治疗中具有重要意义的植物源化合物。本研究的目的是评估用大黄素处理的人宫颈癌HeLa细胞溶酶体区室的变化范围。
使用显微镜技术评估溶酶体区室的变化。为了评估溶酶体酶的活性和溶酶体膜的通透性，采用了分光光度技术。
大黄素引起溶酶体区室的显著变化，表现为溶酶体、自噬泡数量增加以及溶酶体水解酶活性增强。大黄素通过溶酶体膜损伤对溶酶体表现出细胞毒性活性，可能导致溶酶体水解酶泄漏到细胞质中。
大黄素通过一种明显涉及溶酶体区室的机制诱导降解过程并促进肿瘤细胞死亡。
