培养在百日咳博德特氏菌感染实验室诊断中的重要性。
Importance of culture in laboratory diagnosis of Bordetella pertussis infections.
作者信息
Gilligan P H, Fisher M C
出版信息
J Clin Microbiol. 1984 Nov;20(5):891-3. doi: 10.1128/jcm.20.5.891-893.1984.
Abstract
Nasopharyngeal secretions from 223 patients were examined for the presence of Bordetella pertussis and B. parapertussis by culturing on Regan-Lowe agar (J. Regan and F. Lowe, J. Clin. Microbiol. 6:303-309, 1977) and by direct fluorescent-antibody testing. B. pertussis was found in 38 patients; B. parapertussis was recovered from 2. Culturing was more sensitive (38 of 38 patients) than direct fluorescent-antibody testing (26 of 38 patients) for the detection of B. pertussis. Overgrowth by other organisms (7 of 223 patients) was uncommon. The patients with B. pertussis infections were generally less than 1 year old, had received no or one immunization, and had coughing spells but infrequently had whooping cough. Accurate diagnosis of B. pertussis infections should include culturing.
摘要
通过在Regan-Lowe琼脂培养基上培养（J. Regan和F. Lowe，《临床微生物学杂志》6:303 - 309，1977年）以及直接荧光抗体检测，对223例患者的鼻咽分泌物进行了百日咳博德特氏菌和副百日咳博德特氏菌检测。在38例患者中发现了百日咳博德特氏菌；从2例患者中分离出了副百日咳博德特氏菌。对于百日咳博德特氏菌的检测，培养法（38例患者中的38例）比直接荧光抗体检测法（38例患者中的26例）更敏感。其他微生物过度生长（223例患者中的7例）并不常见。百日咳博德特氏菌感染患者一般年龄小于1岁，未接种或仅接种过一剂疫苗，有咳嗽发作，但很少有百日咳。百日咳博德特氏菌感染的准确诊断应包括培养。
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