用于对致病性大肠杆菌菌毛进行血清分型的单克隆抗体。
Monoclonal antibodies for serotyping the P fimbriae of uropathogenic Escherichia coli.
作者信息
de Ree J M, Schwillens P, van den Bosch J F
出版信息
J Clin Microbiol. 1986 Jul;24(1):121-5. doi: 10.1128/jcm.24.1.121-125.1986.
Abstract
Monoclonal antibodies (MAbs) against seven serologically different P fimbriae (F7(1), F7(2), F8, F9, F11, F12, and F13) of uropathogenic Escherichia coli were tested for their ability to detect the P fimbriae on wild-type strains. In a plate agglutination test the MABs could detect the fimbriae on strains which expressed cloned fimbriae but not on wild-type strains. In a coagglutination test and in a whole-bacterium enzyme-linked immunosorbent assay the MAbs recognized the fimbriae on strains with cloned fimbriae and on wild-type strains. However, the coagglutination test has some disadvantages: only immunoglobulin G MAbs can be used, and the results cannot be read in an objective way. From these results, we concluded that the whole-bacterium enzyme-linked immunosorbent assay is the most convenient method for the determination of P fimbriae on wild-type E. coli strains. With this fast and easy method it is possible to do epidemiological studies on the distribution of P fimbriae among clinical isolates of uropathogenic E. coli and to extend the O:K:H serotype with the F serotype.
摘要
检测了针对尿路致病性大肠杆菌七种血清学不同的P菌毛（F7(1)、F7(2)、F8、F9、F11、F12和F13）的单克隆抗体（MAb）检测野生型菌株上P菌毛的能力。在平板凝集试验中，单克隆抗体能够检测出表达克隆菌毛的菌株上的菌毛，但不能检测野生型菌株上的菌毛。在协同凝集试验和全菌酶联免疫吸附试验中，单克隆抗体能够识别表达克隆菌毛的菌株和野生型菌株上的菌毛。然而，协同凝集试验有一些缺点：只能使用免疫球蛋白G单克隆抗体，并且结果无法客观读取。从这些结果中，我们得出结论，全菌酶联免疫吸附试验是测定野生型大肠杆菌菌株上P菌毛最方便的方法。通过这种快速简便的方法，可以对尿路致病性大肠杆菌临床分离株中P菌毛的分布进行流行病学研究，并将O:K:H血清型扩展为F血清型。
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1
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J Hyg (Lond). 1982 Jun;88(3):567-77. doi: 10.1017/s002217240007042x.
2
Molecular organisation of the genes involved in the production of F7(2) fimbriae, causing mannose-resistant haemagglutination, of a uropathogenic Escherichia coli 06:K2:H1:F7 strain.
Mol Gen Genet. 1984;194(3):528-33. doi: 10.1007/BF00425570.
3
Serology of Escherichia coli fimbriae.大肠杆菌菌毛的血清学
Prog Allergy. 1983;33:80-105.
4
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Infect Immun. 1982 Nov;38(2):739-44. doi: 10.1128/iai.38.2.739-744.1982.
5
P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains.来自人源致病性大肠杆菌菌株的P抗原识别菌毛
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6
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Infect Immun. 1981 Sep;33(3):933-8. doi: 10.1128/iai.33.3.933-938.1981.
7
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8
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9
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10
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