Enteric Diseases Laboratory Branch, Center for Disease Control & Prevention, Atlanta, GA, USA.
Enteric Diseases Laboratory Branch, Center for Disease Control & Prevention, Atlanta, GA, USA.
Clin Microbiol Infect. 2018 Apr;24(4):335-341. doi: 10.1016/j.cmi.2017.10.013. Epub 2017 Oct 23.
With the efficiency and the decreasing cost of next-generation sequencing, the technology is being rapidly introduced into clinical and public health laboratory practice.
The historical background and principles of first-, second- and third-generation sequencing are described, as are the characteristics of the most commonly used sequencing instruments.
Peer-reviewed literature, white papers and meeting reports.
Next-generation sequencing is a technology that could potentially replace many traditional microbiological workflows, providing clinicians and public health specialists with more actionable information than hitherto achievable. Examples of the clinical and public health uses of the technology are provided. The challenge of comparability of different sequencing platforms is discussed. Finally, the future directions of the technology integrating it with laboratory management and public health surveillance systems, and moving it towards performing sequencing directly from the clinical specimen (metagenomics), could lead to yet another fundamental transformation of clinical diagnostics and public health surveillance.
随着下一代测序技术的效率提高和成本降低，该技术正在迅速引入临床和公共卫生实验室实践。
描述第一代、第二代和第三代测序的历史背景和原理，以及最常用测序仪器的特点。
同行评议文献、白皮书和会议报告。
下一代测序技术有可能替代许多传统的微生物学工作流程，为临床医生和公共卫生专家提供比以往任何时候都更多的可操作信息。提供了该技术在临床和公共卫生中的应用实例。讨论了不同测序平台可比性的挑战。最后，该技术与实验室管理和公共卫生监测系统集成的未来方向，以及将其直接从临床标本（宏基因组学）进行测序的方向，可能会导致临床诊断和公共卫生监测的又一次根本性转变。
