Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, El Llano Subercaseaux 2801, San Miguel, Santiago 8900000, Chile.
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad Autónoma de Chile, El Llano Subercaseaux 2801, San Miguel, Santiago 8900000, Chile.
Int J Mol Sci. 2017 Oct 27;18(11):2251. doi: 10.3390/ijms18112251.
The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of with EC values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of , i.e., after six hours of incubation the percentage of germination decreases from 92% (control) to 25% and 5% in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX Green uptake assay. Gene expression studies confirm that the effect of polygodial on is mainly attributed to inhibition of germination and appears at early stages of development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond.
从 Canelo 中提取的次生代谢产物 Polygodial 的抗真菌活性已被评估对不同分离株的菌丝生长的影响。结果表明，Polygodial 影响正常和耐药分离株的生长，EC 值在 117 至 175ppm 之间。此外，Polygodial 明显降低了 的萌发，即在孵育六小时后，萌发率从对照的 92%分别降低至 20ppm 和 80ppm 存在时的 25%和 5%。形态学研究表明，与对照中的分生孢子相比，用 Polygodial 处理的分生孢子更小，细胞膜边界不规则，并且有大量细胞碎片。SYTOX Green 摄取测定证实了 Polygodial 诱导的膜损伤的存在。基因表达研究证实，Polygodial 对 的影响主要归因于萌发的抑制，并且在 的发育早期阶段出现。另一方面，结构上与 Polygodial 非常相似的二烯醇也能有效抑制菌丝生长。因此，这两种化合物通过不同的机制抑制菌丝生长。根据双键上的电子密度讨论了这些化合物的不同抗真菌活性。
