Department of Molecular Sciences, Macquarie University, Sydney, NSW2113, Australia.
Sanitarium Health and Wellbeing, Sydney, NSW2261, Australia.
Sci Rep. 2017 Oct 30;7(1):14312. doi: 10.1038/s41598-017-14707-z.
The introduction of different nutrient and energy sources during weaning leads to significant changes in the infant gut microbiota. We used an in vitro infant digestive and gut microbiota model system to investigate the effect of four commercially available cereal products based on either wheat, sorghum, rice or oats, on the gut microbiota of six infants. Our results indicated cereal additions induced numerous changes in the gut microbiota composition. The relative abundance of bacterial families associated with fibre degradation, Bacteroidaceae, Bifidobacteriaceae, Lactobacillaceae, Prevotellaceae, Ruminococcaceae and Veillonellaceae increased, whilst the abundance of Enterobacteriaceae decreased with cereal additions. Corresponding changes in the production of SCFAs showed higher concentrations of acetate following all cereal additions, whilst, propionate and butyrate varied between specific cereal additions. These cereal-specific variations in the concentrations of SCFAs showed a moderate correlation with the relative abundance of potential SCFA-producing bacterial families. Overall, our results demonstrated clear shifts in the abundance of bacterial groups associated with weaning and an increase in the production of SCFAs following cereal additions.
在断奶期间引入不同的营养和能量来源会导致婴儿肠道微生物群发生显著变化。我们使用体外婴儿消化和肠道微生物群模型系统，研究了基于小麦、高粱、大米或燕麦的四种市售谷物产品对 6 名婴儿肠道微生物群的影响。结果表明，谷物添加物诱导了肠道微生物群组成的许多变化。与纤维降解相关的细菌家族的相对丰度增加，如梭菌科、双歧杆菌科、乳杆菌科、普雷沃氏菌科、瘤胃球菌科和韦荣氏球菌科，而肠杆菌科的丰度随着谷物的添加而减少。相应的 SCFA 产量变化表明，所有谷物添加后乙酸盐浓度升高，而丙酸和丁酸盐在特定谷物添加之间变化。这些 SCFA 的谷物特异性浓度变化与潜在的 SCFA 产生细菌家族的相对丰度呈中度相关。总的来说，我们的结果表明，与断奶相关的细菌群的丰度明显发生变化，并且在谷物添加后 SCFA 的产生增加。
