Peng Jing, Wang Hai-Cheng, Liu Yang, Jiang Jiu-Hui, Lv Wan-Qi, Yang Yue, Li Cui-Ying, Qiu Xiao-Yan
Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China.
Department of Oral Pathology, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China.
Oncol Lett. 2017 Oct;14(4):4491-4498. doi: 10.3892/ol.2017.6782. Epub 2017 Aug 21.
Cancer cell-derived immunoglobulin G (cancer-IgG) has been implicated in the pathogenesis and progression of various types of cancer. However, its role in salivary adenoid cystic carcinoma (SACC) remains unclear. The present study aimed to investigate the effects of cancer-IgG on metastasis and prognosis in 96 patients with SACC. Immunohistochemical staining showed that cancer-IgG expression was present in all 96 individual SACC tissues. Additionally, high cancer-IgG expression was significantly correlated with metastasis, nerve invasion and recurrence in SACC (P<0.05). Moreover, cancer-IgG expression was significantly correlated with the survival duration of patients with SACC (P<0.05). Proliferation, cell motility and invasion all decreased significantly following knockdown of cancer-IgG in SACC cells (P<0.05) through population-doubling time, wound healing and transwell invasion assays. Additionally, cancer-IgG-knockdown in SACC cells induced the increased expression of E-cadherin and matrix metalloproteinase 9, and promoted the epithelial-mesenchymal transition, but decreased the expression of F-actin filaments. Taken together, these results showed that the high expression of cancer-IgG was strongly associated with metastasis, recurrence and invasion in SACC, suggesting that cancer-IgG expression could serve as a useful biomarker to predict the prognosis of the disease.
癌细胞衍生的免疫球蛋白G（癌源性IgG）与多种类型癌症的发病机制和进展有关。然而，其在涎腺腺样囊性癌（SACC）中的作用仍不清楚。本研究旨在探讨癌源性IgG对96例SACC患者转移和预后的影响。免疫组织化学染色显示，所有96个SACC个体组织中均存在癌源性IgG表达。此外，癌源性IgG高表达与SACC的转移、神经侵犯和复发显著相关（P<0.05）。而且，癌源性IgG表达与SACC患者的生存时间显著相关（P<0.05）。通过群体倍增时间、伤口愈合和Transwell侵袭试验，在SACC细胞中敲低癌源性IgG后，增殖、细胞运动性和侵袭均显著降低（P<0.05）。此外，SACC细胞中癌源性IgG敲低诱导E-钙黏蛋白和基质金属蛋白酶9表达增加，促进上皮-间质转化，但降低F-肌动蛋白丝的表达。综上所述，这些结果表明癌源性IgG的高表达与SACC的转移、复发和侵袭密切相关，提示癌源性IgG表达可作为预测该疾病预后的有用生物标志物。
