Proungvitaya Siriporn, Sombattheera Sutthikan, Boonsiri Patcharee, Limpaiboon Temduang, Wongkham Sopit, Wongkham Chaisiri, Titapun Attapol, Proungvitaya Tanakorn
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand.
Oncol Lett. 2017 Oct;14(4):4943-4948. doi: 10.3892/ol.2017.6763. Epub 2017 Aug 17.
Cholangiocarcinoma (CCA) is a cancer of biliary epithelial cell origin, which is prevalent in northeastern Thailand. The majority of patients with CCA are diagnosed at the advanced-stage of the disease. Although the early detection and diagnosis of CCA is critical to improve the prognosis of patients, there are presently no specific tumor markers for CCA. A previous study demonstrated that the total serum bile acid (TSBA) levels of patients with CCA are significantly increased, compared with those of healthy controls. In addition, although statistically insignificant, the TSBA levels in the sera of patients with CCA tended to be increased, as compared with the sera of patients with benign biliary disease (BBD). In the present study, the high performance liquid chromatographic (HPLC) patterns of bile acid composition were compared in the sera of patients with CCA, patients with BBD and normal controls. The results revealed that serum bile acid patterns in patients with CCA varied, compared with those in patients with BBD and normal controls. As hypothesized, glycocholic acid (GCA) levels in the sera of patients with CCA and BBD were high, compared with those in healthy controls. In addition, GCA levels in the sera of patients with CCA tended to be higher, as compared with patients with BBD; however, this result was not statistically significant. Therefore, determination of the bile acid patterns and GCA levels in sera using HPLC is feasible, and may aid the diagnosis of CCA.
胆管癌（CCA）是一种起源于胆管上皮细胞的癌症，在泰国东北部较为常见。大多数CCA患者在疾病晚期才被诊断出来。尽管CCA的早期检测和诊断对于改善患者预后至关重要，但目前尚无针对CCA的特异性肿瘤标志物。先前的一项研究表明，与健康对照组相比，CCA患者的血清总胆汁酸（TSBA）水平显著升高。此外，尽管无统计学意义，但与良性胆道疾病（BBD）患者的血清相比，CCA患者血清中的TSBA水平有升高趋势。在本研究中，对CCA患者、BBD患者和正常对照者血清中胆汁酸组成的高效液相色谱（HPLC）图谱进行了比较。结果显示，与BBD患者和正常对照者相比，CCA患者的血清胆汁酸图谱有所不同。正如所假设的，与健康对照组相比，CCA患者和BBD患者血清中的甘氨胆酸（GCA）水平较高。此外，与BBD患者相比，CCA患者血清中的GCA水平有升高趋势；然而，这一结果无统计学意义。因此，使用HPLC测定血清中的胆汁酸图谱和GCA水平是可行的，可能有助于CCA的诊断。
