茶树两个黄烷酮-3-羟化酶基因的功能分析：在类黄酮积累中的关键作用

Functional Analysis of Two Flavanone-3-Hydroxylase Genes from Camellia sinensis: A Critical Role in Flavonoid Accumulation.

作者信息

Han Yahui, Huang Keyi, Liu Yajun, Jiao Tianming, Ma Guoliang, Qian Yumei, Wang Peiqiang, Dai Xinlong, Gao Liping, Xia Tao

机构信息

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, Anhui, China.

School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China.

出版信息

Genes (Basel). 2017 Oct 31;8(11):300. doi: 10.3390/genes8110300.

Flavonoids are major secondary metabolites in . Flavanone-3-hydroxylase (F3H) is a key enzyme in flavonoid biosynthesis in plants. However, its role in the flavonoid metabolism in has not been well studied. In this study, we cloned two s from , named and , where containing 1107 bases encoded 368 amino acids, and containing 1071 bases encoded 357 amino acids. Enzymatic activity analysis showed both recombinant CsF3H enzymes in could convert naringenin and eriodictyol into dihydrokaempferol (DHK) and dihydroquercetin (DHQ), respectively. The expression profiles showed that and were highly expressed in the tender leaves of tea plants. Under different abiotic stresses, the two s were induced remarkably by ultraviolet (UV) radiation, sucrose, and abscisic acid (ABA). In the seeds of s transgenic , the concentration of most flavonol glycosides and oligomeric proanthocyanidins increased significantly, while the content of monocatechin derivatives decreased. The present study revealed that s played critical roles in flavonoid biosynthesis in tea plants.

类黄酮是……中的主要次生代谢产物。黄烷酮-3-羟化酶（F3H）是植物类黄酮生物合成中的关键酶。然而，其在……类黄酮代谢中的作用尚未得到充分研究。在本研究中，我们从……中克隆了两个基因，命名为……和……，其中……含有1107个碱基，编码368个氨基酸，……含有1071个碱基，编码357个氨基酸。酶活性分析表明，……中的两种重组CsF3H酶均可分别将柚皮素和圣草酚转化为二氢山奈酚（DHK）和二氢槲皮素（DHQ）。表达谱显示，……和……在茶树嫩叶中高表达。在不同非生物胁迫下，这两个基因受到紫外线（UV）辐射、蔗糖和脱落酸（ABA）的显著诱导。在……转基因……的种子中，大多数黄酮醇糖苷和原花青素低聚物的浓度显著增加，而单儿茶素衍生物的含量降低。本研究表明，……基因在茶树类黄酮生物合成中起关键作用。