State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, 330045, Nanchang, China.
Sci Rep. 2017 Nov 2;7(1):14928. doi: 10.1038/s41598-017-15044-x.
Serum glucose and lipids are important indicators for host metabolic condition. Interaction of host and gut microbes regulates the metabolism process. However, how much the gut microbiome contributes to the variance of serum glucose and lipids is largely unknown. Here we carried out a 16S rRNA gene based association study between cecum microbiome and the concentration of serum glucose and lipids in 240 Chinese Erhualian pigs. We identified tens of bacterial taxa associated with serum glucose and lipids. The butyrate-producing bacteria were significantly associated with serum glucose level. The pathogenic bacteria belonging to Proteobacteria and Fusobacteria showed significant associations with increased serum lipid levels, while the bacteria Lactobacillus and Bacilli had negative correlations with serum lipids. Cross-validation analysis revealed that 23.8% variation of serum glucose and 1.6%~6.0% variations of serum lipids were explained by gut microbiome. Furthermore, predicted function capacities related to nutrition intake, transport and carbohydrate metabolism were significantly associated with serum glucose level, while the pathways related to antioxidant metabolism and bile synthesis tended to be associated with serum lipid level. The results provide meaningful information to get insight into the effect of gut microbiome on serum glucose and lipid levels in pigs.
血清葡萄糖和脂质是宿主代谢状况的重要指标。宿主与肠道微生物的相互作用调节着代谢过程。然而，肠道微生物组对血清葡萄糖和脂质变化的贡献程度在很大程度上尚不清楚。在这里，我们对 240 头中国二元猪的盲肠微生物组与血清葡萄糖和脂质浓度之间进行了基于 16S rRNA 基因的关联研究。我们鉴定出数十种与血清葡萄糖和脂质相关的细菌分类群。产生丁酸的细菌与血清葡萄糖水平显著相关。属于变形菌门和梭杆菌门的致病菌与血清脂质水平升高显著相关，而乳杆菌属和芽孢杆菌属的细菌与血清脂质呈负相关。交叉验证分析显示，肠道微生物组可以解释 23.8%的血清葡萄糖变化和 1.6%~6.0%的血清脂质变化。此外，与营养摄入、运输和碳水化合物代谢相关的预测功能能力与血清葡萄糖水平显著相关，而与抗氧化代谢和胆汁合成相关的途径则与血清脂质水平相关。这些结果为深入了解肠道微生物组对猪血清葡萄糖和脂质水平的影响提供了有意义的信息。
