Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
J Appl Microbiol. 2012 Apr;112(4):623-30. doi: 10.1111/j.1365-2672.2012.05233.x. Epub 2012 Feb 20.
To determine the prevalence of carriage of methicillin-resistant Staphylococcus pseudintermedius (MRSP) among dogs with pyoderma from two small animal hospitals in North China during a 21-month period and to characterize these isolates.
Swabs were taken from 260 dogs with pyoderma, and the staphylococcal species isolated and methicillin resistance were confirmed phenotypically and genotypically. The identified MRSP isolates were characterized by multilocus sequence typing (MLST), spa typing, staphylococcal cassette chromosome (SCC) mec typing, testing for susceptibility to nine antimicrobial agents and SmaI-digested pulsed-field gel electrophoresis. Thirty-three (12·7%) dogs were positive for MRSP. The most prevalent genotypes detected among MRSP were ST71(MLST)-t06(spa)-II-III(SCCmec) (n = 22, 66·7%), followed by ST5-t19 (n = 8, 24·2%), ST126-III(n = 2, 6·1%) and ST6-t02-V (3·0%). All MRSP isolates showed extended resistance to tested antimicrobial agents. Eight different SmaI patterns were observed in 21 typeable MRSP isolates.
Clinical isolates of MSRP isolated from dogs in North China belonged to two major clonal lineages ST71 and ST5.
This study is the first report on MRSP from canine pyoderma in China. Further surveillance study is needed to gain more detailed data concerning this major clinical challenge in veterinary medicine.
在华北地区的两家小动物医院，通过为期 21 个月的时间，确定患有脓皮病的犬携带耐甲氧西林金黄色葡萄球菌（MRSP）的流行率，并对这些分离株进行特征描述。
从 260 只患有脓皮病的犬中采集拭子，通过表型和基因方法确认分离出的葡萄球菌物种和耐甲氧西林情况。通过多位点序列分型（MLST）、spa 分型、葡萄球菌盒式染色体（SCC） mec 分型、对 9 种抗菌药物的敏感性测试和 SmaI 酶切脉冲场凝胶电泳对鉴定出的 MRSP 分离株进行特征描述。33 只（12.7%）犬对 MRSP 呈阳性。在 MRSP 中检测到的最常见基因型是 ST71（MLST）-t06（spa）-II-III（SCCmec）（n = 22，66.7%），其次是 ST5-t19（n = 8，24.2%）、ST126-III（n = 2，6.1%）和 ST6-t02-V（3.0%）。所有 MRSP 分离株均对测试的抗菌药物表现出广泛的耐药性。在 21 个可分型的 MRSP 分离株中观察到 8 种不同的 SmaI 模式。
来自中国北方犬脓皮病的临床分离株属于 ST71 和 ST5 两个主要克隆谱系。
本研究是中国首例关于犬脓皮病 MRSP 的报道。需要进一步的监测研究，以获得有关兽医临床主要挑战的更详细数据。
