Jain Vardhaman Mulchand, Karibasappa Gundabaktha Nagappa, Dodamani Arun Suresh, Mali Gaurao Vasant
Department of Public Health Dentistry, ACPM Dental College, Dhule, Maharashtra, India.
Department of Public Health Dentistry, Ajeenkya DY Patil Dental School, Pune, Maharashtra, India.
J Educ Health Promot. 2017 Oct 4;6:90. doi: 10.4103/jehp.jehp_41_17. eCollection 2017.
Due to consumption of various forms of tobacco in large amounts by Indian population, it has become a cause of concern for major oral diseases. In 2008, the WHO named tobacco as the world's single greatest cause of preventable death. It is also known that certain amount of carbohydrates are incorporated in processed tobacco to make it acceptable for consumption. Thus, its role in oral diseases becomes an important question at this point of time. Through this study, it is attempted to find out the carbohydrate content of various forms of tobacco by phenol-sulfuric acid method.
Tobacco products selected for the study were Nandi hookah tambakhu (A), photo brand budhaa Punjabi snuff (B), Miraj (C), Gai-chhap tambakhu (D), Hanuman-chhap Pandharpuri tambakhu (E), and Hathi-chhap Bidi (F). The samples were decoded and transported to laboratory and tested at various concentrations by phenol-sulfuric acid method followed by ultraviolet spectrophotometry to determine their absorbance.
The present study showed Hathi-chhap bidi/sample F had a maximum absorbance (1.995) at 10 μg/ml which is a smoking form of tobacco followed by rest all smokeless forms of tobacco, i.e. sample C (0.452), sample B (0.253), sample D (0.077), sample E (-0.018), and sample A (-0.127), respectively.
As the concentration of tobacco sample increases, their absorbance increases which in turn is suggestive of increase in its carbohydrate concentration. Carbohydrates in the form of sugars, either inherently present or added in it during manufacturing can serve as a risk factor for higher incidence of dental caries.
由于印度人口大量消费各种形式的烟草，它已成为主要口腔疾病的一个令人担忧的原因。2008年，世界卫生组织将烟草列为世界上可预防死亡的单一最大原因。还已知在加工烟草中掺入了一定量的碳水化合物以使其适合消费。因此，在这个时候它在口腔疾病中的作用就成为一个重要问题。通过本研究，试图用苯酚 - 硫酸法找出各种形式烟草的碳水化合物含量。
本研究选择的烟草制品有南迪水烟烟草（A）、照片品牌布达哈旁遮普鼻烟（B）、米拉杰（C）、盖 - 查普烟草（D）、哈努曼 - 查普潘德尔普里烟草（E）和哈蒂 - 查普比迪烟（F）。对样品进行编码并运至实验室，通过苯酚 - 硫酸法然后用紫外分光光度法在不同浓度下进行测试以确定其吸光度。
本研究表明，哈蒂 - 查普比迪烟/样品F在10μg/ml时具有最大吸光度（1.995），它是一种吸烟形式的烟草，其次是所有其他无烟形式的烟草，即样品C（0.452）、样品B（0.253）、样品D（0.077）、样品E（ - 0.018）和样品A（ - 0.127）。
随着烟草样品浓度的增加，其吸光度增加，这反过来表明其碳水化合物浓度增加。以糖的形式存在的碳水化合物，无论是天然存在的还是在制造过程中添加的，都可能成为龋齿发病率较高的一个风险因素。
