Tomaskovic-Crook Eva, Crook Jeremy M
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Wollongong, NSW, Australia.
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.
Methods Mol Biol. 2019;1576:13-22. doi: 10.1007/7651_2017_95.
Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined, and reliable for reproducible and scalable organoid generation, and amendable to principles of current good laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing practice (cGMP) toward clinical compliance.
人脑类器官为生物医学研究、转化药物发现、毒理学和组织替代提供了生成三维（3D）类脑组织的机会。在此，我们描述了一种从人诱导多能干细胞（iPSC）快速且明确地诱导生成脑类器官的方案，该方案使用市售的培养和分化培养基以及一种廉价、易于操作且经临床批准的半合成水凝胶。重要的是，该方法简单、明确且可靠，可用于可重复和可扩展的类器官生成，符合现行良好实验室规范（cGLP）原则，并且有可能前瞻性地适用于现行良好生产规范（cGMP）以实现临床合规。
