Danilova Tatiana V, Friebe Bernd, Gill Bikram S, Poland Jesse, Jackson Eric
Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
General Mills NTS [AI]2 Lab, Minneapolis, MN, 55426, USA.
Theor Appl Genet. 2018 Feb;131(2):377-388. doi: 10.1007/s00122-017-3008-z. Epub 2017 Nov 10.
A complete set of six compensating Robertsonian translocation chromosomes involving barley chromosome 7H and three chromosomes of hexaploid wheat was produced. Grain β-glucan content increased in lines containing 7HL. Many valuable genes for agronomic performance, disease resistance and increased yield have been transferred from relative species to wheat (Triticum aestivum L.) through whole-arm Robertsonian translocations (RobT). Although of a great value, the sets of available translocations from barley (Hordeum vulgare L.) are limited. Here, we present the production of a complete set of six compensating RobT chromosomes involving barley chromosome 7H and three group-7 chromosomes of wheat. The barley group-7 long-arm RobTs had a higher grain β-glucan content compared to the wheat control. The β-glucan levels varied depending on the temperature and were higher under hot conditions. Implicated in this increase, the barley cellulose synthase-like F6 gene (CslF6) responsible for β-glucan synthesis was physically mapped near the centromere in the long arm of barley chromosome 7H. Likewise, wheat CslF6 homoeologs were mapped near the centromere in the long arms of all group-7 wheat chromosomes. With the set of novel wheat-barley translocations, we demonstrate a valuable increase of β-glucan, along with a resource of genetic stocks that are likely to carry many other important genes from barley into wheat.
产生了一套完整的六条补偿性罗伯逊易位染色体，涉及大麦7H染色体和六倍体小麦的三条染色体。含有7HL的株系中籽粒β-葡聚糖含量增加。许多具有重要农艺性状、抗病性和增产作用的宝贵基因已通过全臂罗伯逊易位（RobT）从近缘物种转移到小麦（普通小麦）中。尽管具有很高的价值，但来自大麦（大麦）的可用易位系却很有限。在此，我们展示了一套完整的六条补偿性罗伯逊易位染色体的产生，涉及大麦7H染色体和小麦的三条7组染色体。与小麦对照相比，大麦7组长臂罗伯逊易位系的籽粒β-葡聚糖含量更高。β-葡聚糖水平因温度而异，在高温条件下更高。与此增加相关的是，负责β-葡聚糖合成的大麦纤维素合酶样F6基因（CslF6）在大麦7H染色体长臂的着丝粒附近进行了物理定位。同样，小麦CslF6同源基因在所有小麦7组染色体长臂的着丝粒附近进行了定位。利用这套新的小麦-大麦易位系，我们证明了β-葡聚糖的显著增加，以及一种可能携带许多其他来自大麦的重要基因进入小麦的遗传资源。
