Matsumoto H, Sasaki Y
Biochemical Research Lab, Asahi Chemical Industry, Nobeoka, Japan.
Biochem Biophys Res Commun. 1989 Jan 16;158(1):105-9. doi: 10.1016/s0006-291x(89)80183-4.
Staurosporine (10 ng/ml and more), a protein kinase C inhibitor, inhibited the serum-stimulated growth of smooth muscle cells. This inhibitory effect proved to be linked to the inhibition of transition from the G0 to the S phase of the cell cycle, as measured by 5-bromodeoxyuridine incorporation into the nuclei. As this inhibitory effect of staurosporine was restricted to the first 10 hours of the stimulation, reactions which are essential for the signal transduction may be associated with actions of protein kinase C. The possibility that staurosporine may inhibit cell growth via inhibition of this step warrants further attention.
星形孢菌素（10纳克/毫升及以上），一种蛋白激酶C抑制剂，可抑制血清刺激的平滑肌细胞生长。通过将5-溴脱氧尿苷掺入细胞核来测量，这种抑制作用被证明与细胞周期从G0期到S期的转变受到抑制有关。由于星形孢菌素的这种抑制作用仅限于刺激的前10小时，因此对于信号转导至关重要的反应可能与蛋白激酶C的作用有关。星形孢菌素可能通过抑制这一步骤来抑制细胞生长，这一可能性值得进一步关注。
