Institute of Animal Nutrition and Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Sichuan Agricultural University, Ya'an, PR China.
Institute of Animal Nutrition and Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Sichuan Agricultural University, Ya'an, PR China.
Nutrition. 2018 Jan;45:76-84. doi: 10.1016/j.nut.2017.03.012. Epub 2017 Apr 19.
Maternal oxidative stress is harmful for embryonic, fetal, and placental development. The aim of this study was to investigate whether methyl donor supplementation during gestation effectively ameliorates maternal and placenta oxidative stress up to offspring.
Fifty-six Landrace × Yorkshire sows were randomly allocated to receive one of the following four diets during gestation: control diet (CON); control diet supplemented with methyl donor (MET); control diet supplemented with bisphenol A (BPA); and control diet supplemented with BPA and MET (BPA + MET). Blood sample, chorioallantois, and piglets' liver samples were analyzed for antioxidant status and mRNA expression of genes regarding oxidative status.
MET diets lowered homocysteine concentration in the plasma of sows. They improved activity of antioxidant enzymes in chorioallantois and piglet plasma and liver (catalase, glutathione peroxidase, and total antioxidant capacity; P < 0.01), and also upregulated mRNA expression of copper, zinc-superoxide dismutase (P < 0.01), and glutathione peroxidase 1 (P < 0.05) in the chorioallantois and catalase (P < 0.01) in piglet liver compared with the control group. In contrast, BPA diets increased malondialdehyde (P < 0.01) levels in sows and piglets and decreased total antioxidant capacity (P < 0.01) concentration in sows and umbilical cord blood plasma, as well as downregulated copper, zinc-superoxide dismutase (P = 0.01) in piglet liver compared with MET group.
BPA diets fed to sows during gestation aggravated oxidative stress status in sows and piglets, whereas the methyl donor diets enhanced antioxidant capacity of sows and piglets and ameliorated oxidative stress induced by BPA.
母体氧化应激对胚胎、胎儿和胎盘发育有害。本研究旨在探讨妊娠期间补充甲基供体是否能有效改善母体和胎盘的氧化应激，进而影响后代。
56 头长白猪×约克夏母猪被随机分配到以下四个妊娠期日粮组之一：对照组（CON）；对照组添加甲基供体（MET）；对照组添加双酚 A（BPA）；对照组添加 BPA 和 MET（BPA+MET）。分析血液样本、绒毛尿囊膜和仔猪肝脏样本的抗氧化状态和氧化状态相关基因的 mRNA 表达。
MET 日粮降低了母猪血浆中的同型半胱氨酸浓度。它们提高了绒毛尿囊膜和仔猪血浆及肝脏中抗氧化酶的活性（过氧化氢酶、谷胱甘肽过氧化物酶和总抗氧化能力；P<0.01），并上调了绒毛尿囊膜中铜锌超氧化物歧化酶（P<0.01）和谷胱甘肽过氧化物酶 1（P<0.05）和仔猪肝脏中过氧化氢酶（P<0.01）的 mRNA 表达，与对照组相比。相比之下，BPA 日粮增加了母猪和仔猪的丙二醛（P<0.01）水平，并降低了母猪和脐带血血浆中的总抗氧化能力（P<0.01），以及仔猪肝脏中的铜锌超氧化物歧化酶（P=0.01），与 MET 组相比。
妊娠期间给母猪饲喂 BPA 日粮加重了母猪和仔猪的氧化应激状态，而甲基供体日粮增强了母猪和仔猪的抗氧化能力，并改善了 BPA 诱导的氧化应激。
