Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan 410125, China.
Food Funct. 2018 Nov 14;9(11):5843-5854. doi: 10.1039/c8fo01343h.
Maternal dietary supplementation during gestation and lactation improves the health of piglets. The purpose of this study was to determine the effect of different levels of methionine (Met) supplementation in the sows' diet during late gestation and lactation on piglets. Thirty sows were randomly divided into three groups and fed the following diets from day 90 of gestation to day 21 of lactation: (a) control group (a basal diet containing 0.36% Met), (b) 0.48% Met group (a basal diet with additional 0.12% Met), and (c) 0.60% Met group (a basal diet with additional 0.24% Met). On day 21 after farrowing, piglets of average body weight (n = 10 per group) were selected for sample collection. The results showed that the 0.48% Met and 0.60% Met diets significantly lowered the malondialdehyde content in the piglets' serum (P < 0.05). In addition, the glutathione peroxidase content was significantly increased in the 0.48% Met group (P < 0.05) and the total glutathione content was significantly reduced in the 0.60% Met group (P < 0.05) compared to the control group. Furthermore, Met supplementation of the sows' diet was associated with alterations in 37 plasma metabolites in the piglets. In the piglets' intestinal microbiota, the relative abundances of Phascolarctobacterium and Bacteroidetes in the 0.48% Met group were higher than those in the other two groups (P < 0.05). Our results suggest that a diet including 0.48% Met during late gestation and lactation can maintain the health of piglets by increasing the antioxidant capacity and changing the intestinal microbiota composition, but a higher level of Met supplementation may increase the potential risk to piglets.
妊娠和哺乳期母体膳食补充剂可改善仔猪健康。本研究旨在确定母猪日粮中不同水平的蛋氨酸（Met）在妊娠后期和哺乳期补充对仔猪的影响。30 头母猪随机分为 3 组，从妊娠第 90 天到哺乳期第 21 天分别饲喂以下日粮：（a）对照组（基础日粮含 0.36%Met）、（b）0.48%Met 组（基础日粮添加 0.12%Met）和（c）0.60%Met 组（基础日粮添加 0.24%Met）。分娩后第 21 天，每组选择平均体重（n=10）的仔猪进行样品采集。结果表明，0.48%Met 和 0.60%Met 日粮显著降低了仔猪血清中丙二醛含量（P＜0.05）。此外，0.48%Met 组谷胱甘肽过氧化物酶含量显著增加（P＜0.05），0.60%Met 组总谷胱甘肽含量显著降低（P＜0.05）与对照组相比。此外，母猪日粮中添加 Met 与仔猪血浆中 37 种代谢物的变化有关。在仔猪肠道微生物群中，0.48%Met 组的 Phascolarctobacterium 和拟杆菌门的相对丰度高于其他两组（P＜0.05）。我们的研究结果表明，妊娠后期和哺乳期添加 0.48%Met 的日粮可以通过提高抗氧化能力和改变肠道微生物群组成来维持仔猪的健康，但更高水平的 Met 补充可能会增加仔猪的潜在风险。
