用于氧化还原信号和氧化应激的化学探针。
Chemical Probes for Redox Signaling and Oxidative Stress.
机构信息
Department of Chemistry, Boston College, Chestnut Hill, Massachusetts.
出版信息
Antioxid Redox Signal. 2019 Apr 1;30(10):1369-1386. doi: 10.1089/ars.2017.7408. Epub 2017 Dec 22.
SIGNIFICANCE
Cellular reactive oxygen species (ROS) mediate redox signaling cascades that are critical to numerous physiological and pathological processes. Analytical methods to monitor cellular ROS levels and proteomic platforms to identify oxidative post-translational modifications (PTMs) of proteins are critical to understanding the triggers and consequences of redox signaling. Recent Advances: The prevalence and significance of redox signaling has recently been illuminated through the use of chemical probes that allow for sensitive detection of cellular ROS levels and proteomic dissection of oxidative PTMs directly in living cells.
CRITICAL ISSUES
In this review, we provide a comprehensive overview of chemical probes that are available for monitoring ROS and oxidative PTMs, and we highlight the advantages and limitations of these methods.
FUTURE DIRECTIONS
Despite significant advances in chemical probes, the low levels of cellular ROS and low stoichiometry of oxidative PTMs present challenges for accurately measuring the extent and dynamics of ROS generation and redox signaling. Further improvements in sensitivity and ability to spatially and temporally control readouts are essential to fully illuminate cellular redox signaling.
意义
细胞活性氧（ROS）介导的氧化还原信号级联对于许多生理和病理过程至关重要。用于监测细胞 ROS 水平的分析方法和鉴定蛋白质氧化翻译后修饰（PTMs）的蛋白质组学平台对于理解氧化还原信号的触发和后果至关重要。
最新进展
最近，通过使用化学探针，能够灵敏地检测细胞 ROS 水平，并直接在活细胞中进行氧化 PTM 的蛋白质组学分析，阐明了氧化还原信号的普遍性和重要性。
关键问题
在这篇综述中，我们全面概述了可用于监测 ROS 和氧化 PTM 的化学探针，并强调了这些方法的优缺点。
未来方向
尽管在化学探针方面取得了重大进展，但细胞 ROS 水平低和氧化 PTM 低化学计量给准确测量 ROS 生成和氧化还原信号的程度和动态带来了挑战。进一步提高灵敏度以及空间和时间控制读数的能力对于充分阐明细胞氧化还原信号至关重要。
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