Lara-Gonzalez Pablo, Kim Taekyung, Desai Arshad
Ludwig Institute for Cancer Research, La Jolla, California 92093.
Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093.
Cold Spring Harb Symp Quant Biol. 2017;82:111-121. doi: 10.1101/sqb.2017.82.033712. Epub 2017 Nov 13.
The anaphase-promoting complex/cyclosome (APC/C) is a large multisubunit ubiquitin ligase that triggers the metaphase-to-anaphase transition in the cell cycle by targeting the substrates cyclin B and securin for destruction. APC/C activity toward these two key substrates requires the coactivator Cdc20. To ensure that cells enter mitosis and partition their duplicated genome with high accuracy, APC/C activity must be tightly controlled. Here, we discuss the mechanisms that regulate APC/C activity both before and during mitosis. We focus our discussion primarily on the chromosomal pathways that both accelerate and delay APC/C activation by targeting Cdc20 to opposing fates. The findings discussed provide an overview of how cells control the activation of this major cell cycle regulator to ensure both accurate and timely cell division.
后期促进复合物/细胞周期体（APC/C）是一种大型多亚基泛素连接酶，它通过靶向细胞周期蛋白B和分离酶等底物进行降解，从而触发细胞周期中从中期到后期的转变。APC/C对这两种关键底物的活性需要共激活因子Cdc20。为确保细胞进入有丝分裂并高精度地分配其复制的基因组，必须严格控制APC/C的活性。在此，我们讨论在有丝分裂之前和期间调节APC/C活性的机制。我们的讨论主要集中在通过将Cdc20导向相反命运来加速和延迟APC/C激活的染色体途径。所讨论的研究结果概述了细胞如何控制这种主要细胞周期调节因子的激活，以确保准确和及时的细胞分裂。
