• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长程 PCR 基础的下一代测序在一般麻醉患者中丙泊酚药代动力学和药效学研究中的应用。

Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia.

机构信息

Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479, Poznan, Poland.

The NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614, Poznan, Poland.

出版信息

Sci Rep. 2017 Nov 13;7(1):15399. doi: 10.1038/s41598-017-15657-2.

DOI:10.1038/s41598-017-15657-2
PMID:29133890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5684313/
Abstract

The individual response of patients to propofol results from the influence of genetic factors. However, the state of knowledge in this matter still remains insufficient. The aim of our study was to determine genetic predictors of variable pharmacokinetics and pharmacodynamics of propofol within selected 9 genes coding for propofol biotransformation enzymes, receptors and transporters. Our studies are the first extensive pharmaocgenetics research of propofol using high throughput sequencing technology. After the design and optimization of long range PCR-based next-generation sequencing experiment, we screened promoter and coding sequences of all genes analyzed among 87 Polish patients undergoing general anaesthesia with propofol. Initially we found that two variants, c.516 G > T in the CYP2B6 gene and c.2677 T > G in the ABCB1 gene, significantly correlate with propofol's metabolic profile, however after Bonferroni correction the P-values were not statistically significant. Our results suggest, that variants within the CYP2B6 and ABCB1 genes correlate stronger with propofol's metabolic profile compared to other 7 genes. CYP2B6 and ABCB1 variants can play a potentially important role in response to this anaesthetic and they are promising object for further studies.

摘要

患者对丙泊酚的个体反应是由遗传因素影响的。然而，在这方面的知识状况仍然不足。我们的研究目的是确定 9 个编码丙泊酚生物转化酶、受体和转运体的基因中，与丙泊酚药代动力学和药效动力学变异性相关的遗传预测因子。我们的研究是使用高通量测序技术对丙泊酚进行的首次广泛的药物遗传学研究。在设计和优化基于长距离 PCR 的下一代测序实验后，我们在 87 名接受丙泊酚全身麻醉的波兰患者中筛选了所有分析基因的启动子和编码序列。最初我们发现，CYP2B6 基因中的 c.516G>T 和 ABCB1 基因中的 c.2677T>G 两个变体与丙泊酚的代谢谱显著相关，但经 Bonferroni 校正后 P 值无统计学意义。我们的结果表明，CYP2B6 和 ABCB1 基因内的变体与丙泊酚的代谢谱相关性比其他 7 个基因更强。CYP2B6 和 ABCB1 变体可能在对这种麻醉剂的反应中发挥重要作用，它们是进一步研究的有前途的对象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/10915c4f1b42/41598_2017_15657_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/fd6d9ea41055/41598_2017_15657_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/6174e57de649/41598_2017_15657_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/10915c4f1b42/41598_2017_15657_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/fd6d9ea41055/41598_2017_15657_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/6174e57de649/41598_2017_15657_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b14e/5684313/10915c4f1b42/41598_2017_15657_Fig3_HTML.jpg

相似文献

1
Longrange PCR-based next-generation sequencing in pharmacokinetics and pharmacodynamics study of propofol among patients under general anaesthesia.长程 PCR 基础的下一代测序在一般麻醉患者中丙泊酚药代动力学和药效学研究中的应用。
Sci Rep. 2017 Nov 13;7(1):15399. doi: 10.1038/s41598-017-15657-2.
2
The effect of UGT1A9, CYP2B6 and CYP2C9 genes polymorphism on individual differences in propofol pharmacokinetics among Polish patients undergoing general anaesthesia.UGT1A9、CYP2B6和CYP2C9基因多态性对波兰全身麻醉患者丙泊酚药代动力学个体差异的影响。
J Appl Genet. 2017 May;58(2):213-220. doi: 10.1007/s13353-016-0373-2. Epub 2016 Nov 8.
3
Pharmacogenetics, plasma concentrations, clinical signs and EEG during propofol treatment.丙泊酚治疗期间的药物遗传学、血药浓度、临床体征及脑电图
Basic Clin Pharmacol Toxicol. 2014 Dec;115(6):565-70. doi: 10.1111/bcpt.12277. Epub 2014 Jul 19.
4
Association of the CYP2B6 c.516G>T polymorphism with high blood propofol concentrations in women from northern Greece.希腊北部女性中CYP2B6基因c.516G>T多态性与高血药浓度丙泊酚的关联
Drug Metab Pharmacokinet. 2014;29(2):215-8. doi: 10.2133/dmpk.dmpk-13-nt-092. Epub 2013 Oct 22.
5
Impact of clinical factors and UGT1A9 and CYP2B6 genotype on inter-individual differences in propofol pharmacokinetics.临床因素以及UGT1A9和CYP2B6基因对丙泊酚药代动力学个体差异的影响。
J Anesth. 2018 Apr;32(2):236-243. doi: 10.1007/s00540-018-2470-3. Epub 2018 Feb 21.
6
Impact of the Cytochrome P450 2B6 (CYP2B6) Gene Polymorphism c.516G>T (rs3745274) on Propofol Dose Variability.细胞色素P450 2B6（CYP2B6）基因多态性c.516G>T（rs3745274）对丙泊酚剂量变异性的影响。
Eur J Drug Metab Pharmacokinet. 2016 Oct;41(5):511-5. doi: 10.1007/s13318-015-0289-y.
7
Effects of Genetic Variants on the Propofol Dose and Response among Jordanian Arabic Patients Undergoing General Anesthesia.遗传变异对接受全身麻醉的约旦阿拉伯患者异丙酚剂量和反应的影响。
Curr Drug Metab. 2023;23(14):1156-1161. doi: 10.2174/1389200224666230130110350.
8
A Case of Delayed Emergence After Propofol Anesthesia: Genetic Analysis.丙泊酚麻醉后苏醒延迟一例：基因分析
A A Case Rep. 2016 Dec 1;7(11):243-246. doi: 10.1213/XAA.0000000000000397.
9
Influence of Potential Gene Polymorphisms on Propofol Dosage Regimen in Patients Undergoing Abdominal Hysterectomy.潜在基因多态性对腹式子宫切除术患者丙泊酚给药方案的影响
Balkan J Med Genet. 2021 Mar 23;23(2):41-48. doi: 10.2478/bjmg-2020-0030. eCollection 2020 Nov.
10
PharmGKB summary: Efavirenz pathway, pharmacokinetics.药物基因组学知识库总结：依非韦伦途径，药代动力学。
Pharmacogenet Genomics. 2015 Jul;25(7):363-76. doi: 10.1097/FPC.0000000000000145.

引用本文的文献

1
The study of different dosages of remazolam combined with sufentanil and propofol on painless gastroscopy: A randomized controlled trial.研究不同剂量雷米唑仑复合舒芬太尼与丙泊酚用于无痛胃镜的效果：一项随机对照试验。
Medicine (Baltimore). 2023 Aug 25;102(34):e34731. doi: 10.1097/MD.0000000000034731.
2
Clinical Importance of Potential Genetic Determinants Affecting Propofol Pharmacokinetics and Pharmacodynamics.影响丙泊酚药代动力学和药效学的潜在遗传决定因素的临床重要性。
Front Med (Lausanne). 2022 Feb 28;9:809393. doi: 10.3389/fmed.2022.809393. eCollection 2022.
3
Influence of Potential Gene Polymorphisms on Propofol Dosage Regimen in Patients Undergoing Abdominal Hysterectomy.

本文引用的文献

1
The effect of UGT1A9, CYP2B6 and CYP2C9 genes polymorphism on individual differences in propofol pharmacokinetics among Polish patients undergoing general anaesthesia.UGT1A9、CYP2B6和CYP2C9基因多态性对波兰全身麻醉患者丙泊酚药代动力学个体差异的影响。
J Appl Genet. 2017 May;58(2):213-220. doi: 10.1007/s13353-016-0373-2. Epub 2016 Nov 8.
2
Impact of the Cytochrome P450 2B6 (CYP2B6) Gene Polymorphism c.516G>T (rs3745274) on Propofol Dose Variability.细胞色素P450 2B6（CYP2B6）基因多态性c.516G>T（rs3745274）对丙泊酚剂量变异性的影响。
Eur J Drug Metab Pharmacokinet. 2016 Oct;41(5):511-5. doi: 10.1007/s13318-015-0289-y.
3
潜在基因多态性对腹式子宫切除术患者丙泊酚给药方案的影响
Balkan J Med Genet. 2021 Mar 23;23(2):41-48. doi: 10.2478/bjmg-2020-0030. eCollection 2020 Nov.
4
NGS study of glucocorticoid response genes in inflammatory bowel disease patients.炎症性肠病患者糖皮质激素反应基因的二代测序研究
Arch Med Sci. 2019 May 5;17(2):417-433. doi: 10.5114/aoms.2019.84470. eCollection 2021.
Propofol and survival: a meta-analysis of randomized clinical trials.
丙泊酚与生存率：随机临床试验的荟萃分析
Acta Anaesthesiol Scand. 2015 Jan;59(1):17-24. doi: 10.1111/aas.12415. Epub 2014 Oct 14.
4
Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer.新一代测序中的长片段PCR：六种酶的比较及在MiSeq测序仪上的评估
Sci Rep. 2014 Jul 18;4:5737. doi: 10.1038/srep05737.
5
Pharmacogenetics, plasma concentrations, clinical signs and EEG during propofol treatment.丙泊酚治疗期间的药物遗传学、血药浓度、临床体征及脑电图
Basic Clin Pharmacol Toxicol. 2014 Dec;115(6):565-70. doi: 10.1111/bcpt.12277. Epub 2014 Jul 19.
6
Association of the CYP2B6 c.516G>T polymorphism with high blood propofol concentrations in women from northern Greece.希腊北部女性中CYP2B6基因c.516G>T多态性与高血药浓度丙泊酚的关联
Drug Metab Pharmacokinet. 2014;29(2):215-8. doi: 10.2133/dmpk.dmpk-13-nt-092. Epub 2013 Oct 22.
7
The impact of genetic factors on response to anaesthetics.遗传因素对麻醉反应的影响。
Adv Med Sci. 2013;58(1):9-14. doi: 10.2478/v10039-012-0065-z.
8
Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing.通过长距离PCR扩增和新一代测序检测候选基因中导致常染色体显性视网膜色素变性的新突变。
Mol Vis. 2013;19:654-64. Epub 2013 Mar 21.
9
Genotype and allele frequencies of polymorphic UGT1A9 in the Polish population.波兰人群中多态性UGT1A9的基因型和等位基因频率。
Eur J Drug Metab Pharmacokinet. 2013 Sep;38(3):217-21. doi: 10.1007/s13318-012-0110-0. Epub 2012 Nov 25.
10
Effects of amino acid substitutions at positions 33 and 37 on UDP-glucuronosyltransferase 1A9 (UGT1A9) activity and substrate selectivity.位置 33 和 37 上氨基酸取代对尿苷二磷酸葡萄糖醛酸转移酶 1A9（UGT1A9）活性和底物选择性的影响。
Biochem Pharmacol. 2012 Dec 1;84(11):1511-21. doi: 10.1016/j.bcp.2012.08.026. Epub 2012 Sep 5.