Elshishka Milka, Lazarova Stela, Radoslavov Georgi, Hristov Peter, Peneva Vlada K
Institute of Biodiversity and Ecosystem Research (IBER), Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.
Zookeys. 2017 Sep 14(697):37-58. doi: 10.3897/zookeys.697.13770. eCollection 2017.
The taxonomic position of the endemic Antarctic species (Loof, 1975), , (= Loof, 1975) is discussed on the basis of morphological study, including SEM, morphometric data, postembryonic observations, and sequence data of 18S rDNA and the D2-D3 expansion fragments of the large subunit rDNA. A number of characters such as the cuticle and stoma structures, including the presence of moderately developed cuticularised ring around the oral aperture, peculiarities of pharynx expansion, size and position of the posterior pair of pharyngeal nuclei, a less complex uterus, and the position of a posterior ventromedian supplement show that this species differs substantially from the other members of the genus . Furthermore, both the 18S and 28S rDNA-based phylogenetic trees of the sequences available in the GenBank formed two distinct clusters with being a part of a well-supported group with species of the genus ; therefore, it is proposed that its taxonomic position should be reconsidered.
基于形态学研究,包括扫描电子显微镜观察、形态测量数据、胚后发育观察以及18S核糖体DNA和大亚基核糖体DNA的D2-D3扩增片段的序列数据,讨论了南极特有物种(Loof,1975), , (= Loof,1975)的分类地位。一些特征,如角质层和气孔结构,包括口孔周围存在适度发育的角质化环、咽扩张的特点、后对咽核的大小和位置、不太复杂的子宫以及后腹中线附属物的位置,表明该物种与该属的其他成员有很大不同。此外,GenBank中可用序列的基于18S和28S核糖体DNA的系统发育树形成了两个不同的聚类, 是一个得到充分支持的类群的一部分,该类群包含该属的物种;因此,建议重新考虑其分类地位。