Yang Peng, Ma Junhong, Yang Xin, Li Wei
Department of Thoracic Surgery, Linyi People's Hospital, Linyi 276000, China.
The Statistics Research and Consulting Laboratory, Culverhouse College of Commerce and Business Administration, The University of Alabama, Tuscaloosa, AL 35487, USA.
Oncotarget. 2017 Jul 18;8(48):83650-83659. doi: 10.18632/oncotarget.19330. eCollection 2017 Oct 13.
To investigate the clinical significance of naïve T cells, memory T cells, CD45RA+CD45RO+ T cells, and naïve/memory ratio in non-small cell lung cancer (NSCLC) patients.
Pretreatment peripheral blood samples from 76 NSCLC patients and 28 age- and sex-matched healthy volunteers were collected and tested for immune cells by flow cytometry. We compared the expression of these immune cells between patients and healthy controls and evaluated their predictive roles for survival in NSCLC by cox proportional hazards model.
Decreased naïve CD4+ T cells, naïve CD8+ T cells, CD4+ naïve/memory ratios and CD4+CD45RA+CD45RO+ T cells, and increased memory CD4+ T cells, were observed in 76 NSCLC patients compared to healthy volunteers. Univariate analysis revealed that elevated CD4+ naïve/memory ratio correlated with prolonged progression-free survival (P=0.013). Multivariate analysis confirmed its predictive role with a hazard ratio of 0.35 (95% confidence interval, 0.19-0.75, P=0.012).
Peripheral CD4+ naïve/memory ratio can be used as a predictive biomarker in NSCLC patients and used to optimize personalized treatment strategies.
探讨初始T细胞、记忆T细胞、CD45RA+CD45RO+ T细胞及初始/记忆比值在非小细胞肺癌（NSCLC）患者中的临床意义。
收集76例NSCLC患者及28例年龄、性别匹配的健康志愿者的预处理外周血样本，采用流式细胞术检测免疫细胞。比较患者与健康对照者这些免疫细胞的表达情况，并通过Cox比例风险模型评估其对NSCLC患者生存的预测作用。
与健康志愿者相比，76例NSCLC患者的初始CD4+ T细胞、初始CD8+ T细胞、CD4+初始/记忆比值及CD4+CD45RA+CD45RO+ T细胞减少，记忆CD4+ T细胞增加。单因素分析显示，CD4+初始/记忆比值升高与无进展生存期延长相关（P=0.013）。多因素分析证实其预测作用，风险比为0.35（95%置信区间，0.19-0.75，P=0.012）。
外周血CD4+初始/记忆比值可作为NSCLC患者的预测生物标志物，用于优化个性化治疗策略。
