Augmented expression levels of lncRNAs and in gastric cancer tissues and their clinical significance.

OBJECTIVES

As the second cause of cancer death, gastric cancer (GC) is one of the eminent dilemmas all over the world, therefore investigating the molecular mechanisms involved in this cancer is pivotal. Unrestricted proliferation is one of the characteristics of cancerous cells, which is due to deficiency in cell regulatory systems. Long non-coding RNAs (lncRNAs) have emerged as critical regulators of the epigenome. lncRNA () is involved in DNA methylation. This lncRNA reduces promoter methylation by interacting with DNA methyltransferase 1. lncRNA (urothelial carcinoma-associated 1) elevates cell proliferation through the PI3K/Akt signaling pathway which has a critical role in cell growth and apoptosis. The aim of this study was to examine the expression of and genes in GC tissues as well as their clinical significance.

MATERIALS AND METHODS

Total RNA extraction, cDNA synthesis, and quantitative real-time PCR were performed for cells and 80 paired GC tissues. Furthermore, clinical relevance of expression was investigated in TCGA cohort data.

RESULTS

Our results showed and over-expression in GC tissues. Furthermore, lncRNAs associations with clinicopathological features were demonstrated both in the current and TCGA cohort. Kaplan-Meier analysis indicated that patients with higher expression had a worse overall survival in the case of pancreatic and lung adenocarcinomas but not other solid cancer types including GC.

CONCLUSION

These data demonstrate and involvement in GC and suggest that these lncRNAs might be useful as diagnostic/ prognostic biomarkers in cancer.