College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China.
Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.
Genes (Basel). 2019 Nov 20;10(12):951. doi: 10.3390/genes10120951.
Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 602267 differentially expressed unigenes (DEUs). Among these DEUs, 31-790 unigenes were classified into 66-786 categories of gene ontology (GO) functional groups, and 24-760 DEUs could be mapped into 54-268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids.
蚜虫会给小麦生产造成严重损失。在中国所有小麦种植区，麦长管蚜是优势种，对包括吡虫啉和毒死蜱在内的多种杀虫剂具有抗性。然而，麦长管蚜对杀虫剂的抗性及其机制仍不清楚。因此，本研究采用转录组分析比较了吡虫啉和毒死蜱处理 15 min、3 h 和 36 h 后，麦长管蚜应激反应基因的表达模式。首先组装和描述了麦长管蚜成虫转录组，然后比较了不同处理时间的样品与对照样品，共发现 602267 个差异表达的 unigenes (DEUs)。这些 DEUs 中，31-790 个 unigenes 被归类为 66-786 个基因本体 (GO) 功能组，24-760 个 DEUs 可以映射到 54-268 个京都基因与基因组百科全书 (KEGG) 途径。最后，选择了 11 个与杀虫剂耐受性相关的 unigenes，通过每个处理的定量实时聚合酶链反应 (qRT-PCR) 来验证相对表达量。qRT-PCR 和 RNA 测序 (RNA-Seq) 的大多数结果一致。本研究结果将有助于对麦长管蚜及其他麦蚜的杀虫剂抗性进行分子研究。
