[细胞外囊泡临床应用的进展与分析方法]
[Progress and analysis methods of clinical application of extracellular vesicles].
作者信息
An Tai-Xue, Zheng Lei
机构信息
Department of Laboratory Medicine, Nanfang Hospital of Southern Medical University, Guangzhou 510515, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Nov 20;37(11):1559-inside back cover. doi: 10.3969/j.issn.1673-4254.2017.11.23.
Abstract
Extracellular vesicles (EVs) are small vesicles released by cells, which contain proteins and miRNA. It is a new research field in recent years. EVs change accordingly in a variety of diseases. These vesicles can sensitively reflect the pathological changes of the body. Compared with tissue biopsy, EVs detection have the advantages of non-invasive, simple sampling and real-time monitoring. EVs are becoming new diagnostic marker. This article reviews the current status and progress of EVs in clinical application.
摘要
细胞外囊泡（EVs）是细胞释放的小囊泡，其中含有蛋白质和微小核糖核酸（miRNA）。这是近年来一个新的研究领域。在多种疾病中，EVs会相应地发生变化。这些囊泡能够灵敏地反映机体的病理变化。与组织活检相比，检测EVs具有非侵入性、采样简单和实时监测等优点。EVs正成为新的诊断标志物。本文综述了EVs在临床应用中的现状和进展。
相似文献
1
[Progress and analysis methods of clinical application of extracellular vesicles].[细胞外囊泡临床应用的进展与分析方法]
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Nov 20;37(11):1559-inside back cover. doi: 10.3969/j.issn.1673-4254.2017.11.23.
2
Extracellular vesicles and encapusulated miRNAs as emerging cancer biomarkers for novel liquid biopsy.细胞外囊泡和封装的微小RNA作为新型液体活检中新兴的癌症生物标志物。
Jpn J Clin Oncol. 2018 Oct 1;48(10):869-876. doi: 10.1093/jjco/hyy120.
3
MicroRNAs in extracellular vesicles: Sorting mechanisms, diagnostic value, isolation, and detection technology.细胞外囊泡中的微小RNA：分选机制、诊断价值、分离及检测技术
Front Bioeng Biotechnol. 2022 Oct 17;10:948959. doi: 10.3389/fbioe.2022.948959. eCollection 2022.
4
EVmiRNA: a database of miRNA profiling in extracellular vesicles.EVmiRNA：细胞外囊泡中 miRNA 谱分析数据库。
Nucleic Acids Res. 2019 Jan 8;47(D1):D89-D93. doi: 10.1093/nar/gky985.
5
Insights into the Diagnostic Potential of Extracellular Vesicles and Their miRNA Signature from Liquid Biopsy as Early Biomarkers of Diabetic Micro/Macrovascular Complications.从液体活检中探索细胞外囊泡及其 miRNA 特征作为糖尿病微/大血管并发症的早期生物标志物的诊断潜力。
Int J Mol Sci. 2017 Sep 14;18(9):1974. doi: 10.3390/ijms18091974.
6
Intercellular communication by extracellular vesicles and their microRNAs in asthma.细胞外囊泡及其微小RNA在哮喘中的细胞间通讯
Clin Ther. 2014 Jun 1;36(6):873-81. doi: 10.1016/j.clinthera.2014.05.006. Epub 2014 Jun 6.
7
Extracellular Vesicles and Their Role in Urologic Malignancies.细胞外囊泡及其在泌尿系统恶性肿瘤中的作用。
Eur Urol. 2016 Aug;70(2):323-31. doi: 10.1016/j.eururo.2016.02.046. Epub 2016 Feb 28.
8
Extracellular Vesicles in Oncology: Progress and Pitfalls in the Methods of Isolation and Analysis.肿瘤细胞外囊泡：分离和分析方法的进展与陷阱。
Biotechnol J. 2019 Jan;14(1):e1700716. doi: 10.1002/biot.201700716. Epub 2018 Jun 14.
9
The small vesicular culprits: the investigation of extracellular vesicles as new targets for cancer treatment.小囊泡“元凶”：细胞外囊泡作为癌症治疗新靶点的研究
Clin Transl Med. 2017 Dec 13;6(1):45. doi: 10.1186/s40169-017-0176-z.
10
Review: Multiplexed profiling of biomarkers in extracellular vesicles for cancer diagnosis and therapy monitoring.综述：用于癌症诊断和治疗监测的细胞外囊泡中生物标志物的多重分析。
Anal Chim Acta. 2021 Aug 29;1175:338633. doi: 10.1016/j.aca.2021.338633. Epub 2021 May 15.
引用本文的文献
1
[Effect of outer membrane vesicles derived from Escherichia coli on proliferation, apoptosis and migration of human neuroblastoma SK-N-SH cells in vitro].[大肠杆菌来源的外膜囊泡对人神经母细胞瘤SK-N-SH细胞体外增殖、凋亡及迁移的影响]
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Mar 20;38(3):334-339. doi: 10.3969/j.issn.1673-4254.2018.03.15.
本文引用的文献
1
Degradation of serum microRNAs during transient storage of serum samples at 4℃.
Ann Clin Biochem. 2018 Jan;55(1):178-180. doi: 10.1177/0004563217704233. Epub 2017 Jun 29.
2
Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation.暴露于电离辐射后血浆外泌体样囊泡中蛋白质和微小RNA含量的定量变化。
Int J Radiat Biol. 2017 Jun;93(6):569-580. doi: 10.1080/09553002.2017.1294772. Epub 2017 Mar 7.
3
Proteomic analysis of urinary extracellular vesicles from high Gleason score prostate cancer.高 Gleason 评分前列腺癌患者尿细胞外囊泡的蛋白质组学分析。
Sci Rep. 2017 Feb 17;7:42961. doi: 10.1038/srep42961.
4
Characterization of Protein Content Present in Exosomes Isolated from Conditioned Media and Urine.对从条件培养基和尿液中分离出的外泌体中蛋白质含量的表征。
Curr Protoc Protein Sci. 2017 Feb 2;87:24.9.1-24.9.12. doi: 10.1002/cpps.23.
5
Differential miRNA expression in pleural effusions derived from extracellular vesicles of patients with lung cancer, pulmonary tuberculosis, or pneumonia.肺癌、肺结核或肺炎患者细胞外囊泡来源的胸腔积液中miRNA的差异表达。
Tumour Biol. 2016 Dec;37:15835–15845. doi: 10.1007/s13277-016-5410-6. Epub 2016 Oct 14.
6
DNA in serum extracellular vesicles is stable under different storage conditions.血清细胞外囊泡中的DNA在不同储存条件下是稳定的。
BMC Cancer. 2016 Sep 23;16(1):753. doi: 10.1186/s12885-016-2783-2.
7
Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer.循环细胞外囊泡上的纤连蛋白作为检测乳腺癌的液体活检手段
Oncotarget. 2016 Jun 28;7(26):40189-40199. doi: 10.18632/oncotarget.9561.
8
Communication by Extracellular Vesicles: Where We Are and Where We Need to Go.细胞外囊泡通讯：我们的现状与未来展望。
Cell. 2016 Mar 10;164(6):1226-1232. doi: 10.1016/j.cell.2016.01.043.
9
Extracellular vesicles for drug delivery.细胞外囊泡用于药物递送。
Adv Drug Deliv Rev. 2016 Nov 15;106(Pt A):148-156. doi: 10.1016/j.addr.2016.02.006. Epub 2016 Feb 27.
10
Integrated Magneto-Electrochemical Sensor for Exosome Analysis.用于外泌体分析的集成磁电化学传感器
ACS Nano. 2016 Feb 23;10(2):1802-9. doi: 10.1021/acsnano.5b07584. Epub 2016 Jan 28.
Zotero 插件