Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea
College of Public Health, Hebei University, Baoding 071002, China
Int J Mol Sci. 2017 Nov 24;18(12):2512. doi: 10.3390/ijms18122512.
This study investigates in vitro targets related to diabetes in 30 herbal extracts from Peru, for the first time, using α-glucosidase, aldose reductase (AR) inhibitory assays and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging assays. Among the 30 herbal extracts, Juss. (HL) was the herb which showed more than 50% inhibition in all assays, presenting 97.2 ± 2.0%, 56.9 ± 5.6%, 81.9 ± 2.5%, and 58.8 ± 4.6% inhibition for the α-glucosidase, AR, DPPH, and ABTS assays, respectively. Finally, six bioactive compounds, namely, protocatechuic acid, chlorogenic acid, caffeic acid, kaempferol 3--glucuronide, quercetin, and kaempferol were identified in HL by offline high-performance liquid chromatography (HPLC). Quercetin exhibited the strongest inhibition in all enzyme assays and the strongest antioxidant activity. The results suggest that HL shows great potential for the complementary treatment of diabetes and its complications.
本研究首次采用α-葡萄糖苷酶、醛糖还原酶（AR）抑制试验以及 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）清除试验，研究了来自秘鲁的 30 种草药提取物中与糖尿病相关的体外靶点。在这 30 种草药提取物中，Juss.（HL）是在所有试验中均显示出超过 50%抑制作用的草药，其对α-葡萄糖苷酶、AR、DPPH 和 ABTS 试验的抑制率分别为 97.2±2.0%、56.9±5.6%、81.9±2.5%和 58.8±4.6%。最后，通过离线高效液相色谱（HPLC）在 HL 中鉴定出了 6 种生物活性化合物，分别为原儿茶酸、绿原酸、咖啡酸、山柰酚 3-O-葡萄糖苷、槲皮素和山柰酚。槲皮素在所有酶试验中表现出最强的抑制作用和最强的抗氧化活性。研究结果表明，HL 具有治疗糖尿病及其并发症的潜力。
