LINC00636 通过靶向 NM23 促进淋巴结转移和宫颈癌。
LINC00636 promotes lymph node metastasis and cervical cancer through targeting NM23.
机构信息
Department of Ultrasound, Neijiang First People's Hospital, Neijiang city, Sichuan Province, 641000, China.
Department of Ultrasound, West China Hospital of Sichuan University, Chengdu City, Sichuan Province, 610041, China.
出版信息
Biosci Rep. 2020 Oct 30;40(10). doi: 10.1042/BSR20200367.
BACKGROUND
Metastasis is a major obstacle in treatment of cervical cancer, and long non-coding RNA (lncRNA) mediated regulatory effect on associated genes expression is found to be involved in metastasis. However, its mechanisms have not been fully elucidated.
MATERIALS AND METHODS
Specimens from patients with cervical cancer metastasis and non-metastasis were used to screen out candidate non-coding RNAs (ncRNAs) and possible downstream targets. And then, effects were determined in vitro and in vivo through knockdown and overexpression techniques.
RESULTS
LINC00636 was significantly higher in serum and solid tumor cells of metastatic cervical cancer patients than non-metastatic patients. And knockdown of LINC00636 significantly suppressed invasion, proliferation of cervical cancer cells. NM23 expression was negatively regulated by LINC00636 and it mediated anti-tumor effects was partially blocked by overexpression of LINC00636.
CONCLUSION
LINC00636 might promote metastasis of cervical cancer cells through inhibiting NM23 expression.
背景
转移是宫颈癌治疗的主要障碍，长链非编码 RNA（lncRNA）对相关基因表达的调控作用被发现与转移有关。然而，其机制尚未完全阐明。
材料和方法
使用宫颈癌转移和非转移患者的标本筛选候选非编码 RNA（ncRNA）和可能的下游靶标。然后，通过敲低和过表达技术在体外和体内确定其效果。
结果
转移性宫颈癌患者的血清和实体肿瘤细胞中 LINC00636 的水平明显高于非转移性患者。LINC00636 的敲低显著抑制了宫颈癌细胞的侵袭和增殖。NM23 的表达受到 LINC00636 的负调控，LINC00636 的过表达部分阻断了其介导的抗肿瘤作用。
结论
LINC00636 可能通过抑制 NM23 的表达促进宫颈癌细胞的转移。
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