19号染色体微小RNA簇特定成员的循环水平与子痫前期的发生有关。
Circulating levels of specific members of chromosome 19 microRNA cluster are associated with preeclampsia development.
作者信息
Martinez-Fierro Margarita L, Garza-Veloz Idalia, Gutierrez-Arteaga Cristina, Delgado-Enciso Ivan, Barbosa-Cisneros Olga Y, Flores-Morales Virginia, Hernandez-Delgadillo Gloria P, Rocha-Pizaña Maria R, Rodriguez-Sanchez Iram P, Badillo-Almaraz Jose I, Ortiz-Rodriguez Jose M, Castañeda-Miranda Rodrigo, Solis-Sanchez Luis O, Ortiz-Castro Yolanda
机构信息
Unidad Academica de Medicina Humana y Ciencias de la Salud, Molecular Medicine Laboratory, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara km. 6, Ejido la Escondida, Zacatecas, CP 98160, Mexico.
School of Medicine, University of Colima, Colima, Mexico.
出版信息
Arch Gynecol Obstet. 2018 Feb;297(2):365-371. doi: 10.1007/s00404-017-4611-6. Epub 2017 Dec 1.
PURPOSE
To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development.
METHODS
Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates.
RESULTS
A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE (P = 0.007).
CONCLUSIONS
Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.
目的
进行血清微小RNA表达谱分析，以鉴定参与子痫前期发生发展的19号染色体微小RNA簇成员。
方法
使用TaqMan低密度阵列板，对发生子痫前期的孕妇（WWD-PE；n = 16）和对照组（n = 18）在妊娠12、16和20周以及子痫前期诊断时的血清19号染色体微小RNA簇微小RNA表达谱进行评估。
结果
共评估了51个19号染色体微小RNA簇成员。循环中的hsa-miRs 512-3p、518f-3p、520c-3p和520d-3p在两组之间存在差异表达（P < 0.05）。与对照组相比，妊娠20周时hsa-miR-518f-3p的血清水平有助于识别轻度子痫前期（P = 0.035）和重度子痫前期（P = 0.007）。
结论
血清hsa-miRs 512-3p、518f-3p、520c-3p和520d-3p在子痫前期孕妇和对照组之间存在差异表达，其在子痫前期发生发展中的作用应进一步研究。
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