Molecular Medicine Laboratory, Unidad Academica de Medicina Humanay Ciencias de la Salud, Universidad Autonoma de Zacatecas, Zacatecas 98160, Mexico.
Cells. 2021 Apr 24;10(5):1003. doi: 10.3390/cells10051003.
microRNAs are important regulators of cell processes and have been proposed as potential preeclampsia biomarkers. We evaluated serum microRNA expression profiling to identify microRNAs involved in preeclampsia development. Serum microRNA expression profiling was evaluated at 12, 16, and 20 weeks of gestation (WG), and at the time of preeclampsia diagnosis. Two groups were evaluated using TaqMan low-density array plates: a control group with 18 normotensive pregnant women and a case group with 16 patients who developed preeclampsia during the follow-up period. Fifty-three circulating microRNAs were differentially expressed between groups ( < 0.05). Compared with controls, hsa-miR-628-3p showed the highest relative quantity values (at 12 WG = 7.7 and at 20 WG = 3.45) and the hsa-miRs -151a-3p and -573 remained differentially expressed from 16 to 20 WG ( < 0.05). Signaling pathways including cancer-related, axon guidance, Neurotrophin, GnRH, VEGF, and B/T cell receptor, were most commonly altered. Further target gene prediction revealed that nuclear factor of activated T-cells 5 gene was included among the transcriptional targets of preeclampsia-modulated microRNAs. Specific microRNAs including hsa-miRs -628-3p, -151a-3p, and -573 were differentially expressed in serum of pregnant women before they developed preeclampsia compared with controls and their participation in the preeclampsia development should be considered.
microRNAs 是细胞过程的重要调节剂，被认为是子痫前期的潜在生物标志物。我们评估了血清 microRNA 表达谱，以鉴定参与子痫前期发展的 microRNAs。在 12、16 和 20 周妊娠（WG）时以及子痫前期诊断时评估血清 microRNA 表达谱。使用 TaqMan 低密度阵列板评估了两组：对照组有 18 名正常血压孕妇，病例组有 16 名在随访期间发生子痫前期的患者。两组之间有 53 个循环 microRNAs 表达存在差异（<0.05）。与对照组相比，hsa-miR-628-3p 的相对定量值最高（在 12 WG = 7.7，在 20 WG = 3.45），hsa-miRs -151a-3p 和 -573 从 16 到 20 WG 仍保持差异表达（<0.05）。包括癌症相关、轴突导向、神经营养素、GnRH、VEGF 和 B/T 细胞受体在内的信号通路最常发生改变。进一步的靶基因预测表明，核因子活化 T 细胞 5 基因是子痫前期调节 microRNAs 的转录靶标之一。在发生子痫前期之前，孕妇血清中特定的 microRNAs，包括 hsa-miRs -628-3p、-151a-3p 和 -573，与对照组相比存在差异表达，它们参与子痫前期的发展应该被考虑。
