Wu Jiannong, Lyu Bin, Gan Tie'er, Wang Lingcong, Zhu Meifei
Department of Critical Care, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China.
Division of Gastroenterology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4655-4662. doi: 10.3892/etm.2017.5159. Epub 2017 Sep 21.
Electroacupuncture (EA) accelerates intestinal functional recovery in sepsis. The present study investigated ghrelin and ghrelin receptor (GSH-R) levels during EA in rats with acute bowel injury (ABI). Rats were grouped into four groups: Sham, ABI, ABI+EA and ABI+GHRA+EA (n=12 per group). ABI was induced by cecal ligation and puncture (CLP). EA on bilateral Zusanli acupoints was performed following CLP. GSH-R blocker (GHRA) was used following CLP but prior to EA for ABI+GHRA+EA rats. Rats were sacrificed 12 h following CLP. Serum ghrelin, tumor necrosis factor-α (TNF-α) and high mobility group box 1 (HMGB1) levels, as well as ghrelin and GSH-R protein expression, water content, pathological changes and myeloperoxidase (MPO) and diamine oxidase (DAO) activities in the bowel tissues, were measured. ABI rats, compared with the sham rats, had significantly lower levels of ghrelin and GSH-R in the serum and bowel tissue, and higher Chiu's score (all P<0.05). The ABI+EA rats, compared with the ABI rats, had significantly reduced serum TNF-α and HMGB1 levels, bowel water content, MPO activity and Chiu's score (all P<0.05), and significantly higher serum ghrelin (121.2±10.7 vs. 86.7±6.4 pg/ml), bowel ghrelin (0.12±0.02 vs. 0.08±0.01), GSH-R (0.05±0.04 vs. 0.03±0.01) and DAO activity (18.74±4.18 vs. 13.52±2.33 U/ml; all P<0.05), indicating an improvement of the intestinal mucosal barrier. GHRA reversed the protective effects of EA. Therefore, EA improved ABI recovery by promoting ghrelin secretion and upregulating GSH-R expression.
电针可加速脓毒症时肠道功能的恢复。本研究调查了急性肠损伤（ABI）大鼠电针治疗期间胃饥饿素及胃饥饿素受体（GSH-R）水平。大鼠分为四组：假手术组、ABI组、ABI+电针组和ABI+胃饥饿素受体拮抗剂（GHRA）+电针组（每组n = 12）。通过盲肠结扎和穿刺（CLP）诱导ABI。CLP术后对双侧足三里穴位进行电针治疗。对于ABI+GHRA+电针组大鼠，CLP术后但在电针治疗前使用GSH-R拮抗剂（GHRA）。CLP术后12小时处死大鼠。检测血清胃饥饿素、肿瘤坏死因子-α（TNF-α）和高迁移率族蛋白B1（HMGB1）水平，以及胃饥饿素和GSH-R蛋白表达、肠组织含水量、病理变化、髓过氧化物酶（MPO）和二胺氧化酶（DAO）活性。与假手术组大鼠相比，ABI组大鼠血清和肠组织中胃饥饿素和GSH-R水平显著降低，Chiu评分更高（均P<0.05）。与ABI组大鼠相比，ABI+电针组大鼠血清TNF-α和HMGB1水平、肠含水量、MPO活性和Chiu评分显著降低（均P<0.05），血清胃饥饿素（121.2±10.7 vs. 86.7±6.4 pg/ml）、肠胃饥饿素（0.12±0.02 vs. 0.08±0.01）、GSH-R（0.05±0.04 vs. 0.03±0.01）和DAO活性（18.74±4.18 vs. 13.52±2.33 U/ml；均P<0.05）显著升高，表明肠黏膜屏障得到改善。GHRA逆转了电针的保护作用。因此，电针通过促进胃饥饿素分泌和上调GSH-R表达改善ABI恢复。
