Mbatha J N, Galappaththi-Arachchige H N, Mtshali A, Taylor M, Ndhlovu P D, Kjetland E F, Baay M F D, Mkhize-Kwitshana Z L
School Laboratory Medicine and Medical Science, University of KwaZulu-Natal, Durban, South Africa.
Department of Biomedical and Clinical Technology, Durban University of Technology, PO Box 1334, Durban, 4000, South Africa.
BMC Res Notes. 2017 Dec 6;10(1):702. doi: 10.1186/s13104-017-3045-3.
Cervical cancer is a major problem in women and it is important to find a suitable and acceptable screening method, especially among young in low-resource areas for future human papillomavirus (HPV) vaccine follow-up investigations. The study sought to test the acceptability of self-sampling as well as the suitability of the specimen collecting devices.
Ninety-eight young women from rural KwaZulu-Natal were enrolled between March and July 2014. Collected genital specimens were transferred to colour indicator cards for HPV detection. Participants answered a questionnaire where they described their experiences with self-sampling. Samples were tested for high-risk HPV using GP5/6+ PCR.
Of the enrolled participants, 91 answered questionnaires and indicated that self-sampling was preferred by 51/91 (56%) women while 40/91 (44%) indicated preference for sampling by a doctor (p = 0.023). The majority, 64% were comfortable using a swab, 22% preferred a brush while 11% were comfortable with both devices. Of the 98 self-sampled specimens 61 were negative for HPV in both specimens while 37 were HPV-positive in either brush or swab. Of the 37, 26 (70%) were HPV-positive in both brush and swab (kappa = 0.743) and 11 (30%) were discordant.
Self-sampling was acceptable to the majority of participants in this rural area. The Dacron swab was the preferred device, and can be used in combination with colour indicator cards for comfortable self-sampling, easy storage and transport of specimens plus detection.
宫颈癌是女性面临的一个主要问题，找到一种合适且可接受的筛查方法很重要，尤其是在资源匮乏地区的年轻女性中，以便未来进行人乳头瘤病毒（HPV）疫苗后续调查。本研究旨在测试自我采样的可接受性以及标本采集装置的适用性。
2014年3月至7月，招募了98名来自夸祖鲁 - 纳塔尔农村地区的年轻女性。采集的生殖道标本被转移到颜色指示卡上进行HPV检测。参与者回答了一份问卷，描述了她们自我采样的经历。使用GP5/6 + PCR对样本进行高危HPV检测。
在登记的参与者中，91人回答了问卷，其中51/91（56%）的女性表示更喜欢自我采样，而40/91（44%）的女性表示更喜欢由医生采样（p = 0.023）。大多数人（64%）使用拭子时感觉舒适，22%更喜欢刷子，11%对两种装置都感觉舒适。在98份自我采样的标本中，61份在两种标本中HPV均为阴性，而37份在刷子或拭子中HPV呈阳性。在这37份中，26份（70%）在刷子和拭子中HPV均为阳性（kappa = 0.743），11份（30%）不一致。
在这个农村地区，大多数参与者接受自我采样。涤纶拭子是首选装置，可与颜色指示卡结合使用，以便进行舒适的自我采样、标本的轻松储存和运输以及检测。
