Genome Sequences Reveal Cryptic Speciation in the Human Pathogen .

is a pathogenic fungus that causes life-threatening lung infections. About 500,000 people are exposed to each year in the United States, and over 60% of the U.S. population has been exposed to the fungus at some point in their life. We performed genome-wide population genetics and phylogenetic analyses with 30 isolates representing four recognized areas where histoplasmosis is endemic and show that the genus is composed of at least four species that are genetically isolated and rarely interbreed. Therefore, we propose a taxonomic rearrangement of the genus. The evolutionary processes that give rise to new pathogen lineages are critical to our understanding of how they adapt to new environments and how frequently they exchange genes with each other. The fungal pathogen provides opportunities to precisely test hypotheses about the origin of new genetic variation. We find that is composed of at least four different cryptic species that differ genetically and also in virulence. These results have implications for the epidemiology of histoplasmosis because not all species are equivalent in their geographic range and ability to cause disease.