Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
University of Chinese Academy of Sciences, Shanghai 201602, China.
J Integr Plant Biol. 2018 May;60(5):369-375. doi: 10.1111/jipb.12620. Epub 2018 Jan 23.
In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) to introduce a loss-of-function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.
在水稻中，直链淀粉含量（AC）受单个显性 Waxy 基因控制。我们使用成簇规律间隔短回文重复序列（CRISPR）/CRISPR 相关蛋白 9（Cas9）在两个广泛种植的优良粳稻品种中对 Waxy 基因进行了功能丧失突变的引入。我们的结果表明，Waxy 基因突变降低了 AC，并将水稻转化为糯稻，而不影响其他理想的农艺性状，为在优良品种中提高糯性提供了一种有效且简单的策略。重要的是，我们成功地从后代中去除了转基因。我们的研究提供了一个通过直接在优良作物品种中编辑目标基因来生成具有商业化潜力的改良作物的例子。
