Castellucci V F, Blumenfeld H, Goelet P, Kandel E R
Laboratory of Neurobiology and Behavior, Clinical Research Institute of Montreal, Quebec, Canada.
J Neurobiol. 1989 Jan;20(1):1-9. doi: 10.1002/neu.480200102.
To study the effects of protein synthesis inhibition on long-term sensitization of the gill- and siphon-withdrawal reflex of Aplysia, we have developed an isolated reflex preparation in which we could expose the inhibitor to only that part of the central nervous system involved in mediating the reflex and not to the other parts of the animal's central nervous system, thus minimizing the possible systemic side effects. We have found that long-term sensitization can be obtained in the isolated gill reflex, and that this long-term process, but not the short-term process, is blocked selectively by anisomycin, a reversible inhibitor of protein synthesis. Moreover, to obtain this blockade of long-term sensitization, this drug need only be applied during the training procedure.
为了研究蛋白质合成抑制对海兔鳃和虹吸管退缩反射长期敏感化的影响，我们开发了一种离体反射制备方法，在此方法中，我们可以仅将抑制剂作用于参与介导该反射的中枢神经系统部分，而不作用于动物中枢神经系统的其他部分，从而将可能的全身副作用降至最低。我们发现，在离体鳃反射中可获得长期敏感化，并且这种长期过程而非短期过程会被茴香霉素（一种蛋白质合成的可逆抑制剂）选择性阻断。此外，为了实现对长期敏感化的这种阻断，该药物只需在训练过程中应用即可。
