Fischedick Justin, Van Der Kooy Frank, Verpoorte Robert
Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, P.O. Box 9502, 2300RA Leiden, the Netherlands.
Chem Pharm Bull (Tokyo). 2010 Feb;58(2):201-7. doi: 10.1248/cpb.58.201.
Cannabis sativa L. (cannabis) extracts, vapor produced by the Volcano vaporizer and smoke made from burning cannabis joints were analyzed by GC-flame ionization detecter (FID), GC-MS and HPLC. Three different medicinal cannabis varieties were investigated Bedrocan, Bedrobinol and Bediol. Cannabinoids plus other components such as terpenoids and pyrolytic by-products were identified and quantified in all samples. Cannabis vapor and smoke was tested for cannabinoid receptor 1 (CB1) binding activity and compared to pure Delta(9)-tetrahydrocannabinol (Delta(9)-THC). The top five major compounds in Bedrocan extracts were Delta(9)-THC, cannabigerol (CBG), terpinolene, myrcene, and cis-ocimene in Bedrobinol Delta(9)-THC, myrcene, CBG, cannabichromene (CBC), and camphene in Bediol cannabidiol (CBD), Delta(9)-THC, myrcene, CBC, and CBG. The major components in Bedrocan vapor (>1.0 mg/g) were Delta(9)-THC, terpinolene, myrcene, CBG, cis-ocimene and CBD in Bedrobinol Delta(9)-THC, myrcene and CBD in Bediol CBD, Delta(9)-THC, myrcene, CBC and terpinolene. The major components in Bedrocan smoke (>1.0 mg/g) were Delta(9)-THC, cannabinol (CBN), terpinolene, CBG, myrcene and cis-ocimene in Bedrobinol Delta(9)-THC, CBN and myrcene in Bediol CBD, Delta(9)-THC, CBN, myrcene, CBC and terpinolene. There was no statistically significant difference between CB1 binding of pure Delta(9)-THC compared to cannabis smoke and vapor at an equivalent concentration of Delta(9)-THC.
采用气相色谱 - 火焰离子化检测器（FID）、气相色谱 - 质谱联用仪（GC - MS）和高效液相色谱法（HPLC）对大麻（Cannabis sativa L.）提取物、Volcano蒸发器产生的蒸汽以及燃烧大麻卷烟产生的烟雾进行了分析。研究了三种不同的药用大麻品种：Bedrocan、Bedrobinol和Bediol。对所有样品中的大麻素以及萜类化合物和热解副产物等其他成分进行了鉴定和定量。测试了大麻蒸汽和烟雾与大麻素受体1（CB1）的结合活性，并与纯Δ⁹ - 四氢大麻酚（Δ⁹ - THC）进行了比较。Bedrocan提取物中的前五大主要化合物为Δ⁹ - THC、大麻二酚（CBG）、萜品油烯、月桂烯和顺式罗勒烯；Bedrobinol中的为Δ⁹ - THC、月桂烯、CBG、大麻色烯（CBC）和莰烯；Bediol中的为大麻二酚（CBD）、Δ⁹ - THC、月桂烯、CBC和CBG。Bedrocan蒸汽中的主要成分（>1.0 mg/g）为Δ⁹ - THC、萜品油烯、月桂烯、CBG、顺式罗勒烯和CBD；Bedrobinol中的为Δ⁹ - THC、月桂烯和CBD；Bediol中的为CBD、Δ⁹ - THC、月桂烯、CBC和萜品油烯。Bedrocan烟雾中的主要成分（>1.0 mg/g）为Δ⁹ - THC、大麻酚（CBN）、萜品油烯、CBG、月桂烯和顺式罗勒烯；Bedrobinol中的为Δ⁹ - THC和CBN以及月桂烯；Bediol中的为CBD、Δ⁹ - THC、CBN、月桂烯、CBC和萜品油烯。在Δ⁹ - THC浓度相当的情况下，纯Δ⁹ - THC与大麻烟雾和蒸汽的CB1结合之间没有统计学上的显著差异。
