He Qiuyan, Zhou Yangying, Fu Chunyan, Zhu Wei, Zhou Jianhua, Liu Shuang, Tao Yongguang, Xiao Desheng
Department of Pathology, Xiangya Hospital/School of Basic Medicine, Central South University, Hunan, China.
Department of Oncology, Xiangya Hospital, Central South University, Hunan, China.
J Cancer Res Ther. 2017;13(5):807-812. doi: 10.4103/jcrt.JCRT_280_17.
The objective of this study is to investigate the histogenesis of lymphoepithelial carcinoma (LEC) and its relationship with Epstein-Barr virus (EBV).
The expression of EBV was detected using in situ hybridization, and the CK5/6, p63, and p40 expression levels were detected using immunohistochemistry in 45 paraffin-embedded tissues from LEC.
In 45 paraffin-embedded LEC tissues from 10 different samples, the positive CK5/6 signals were located in the cell membrane. The positive signals for p63 and p40 were located in the cell nucleus. In all LEC cases, the positive rates of CK5/6, p63, and p40 were 93.3% (42/45), 95.6% (43/45), and 93.3% (42/45), respectively. The positive EBV-encoded RNA (EBER) signals were located in the cell nucleus. In the 45 LEC cases, the expression of EBER was strongly positive with a positive rate of 100% (45/45).
LEC is closely related to EBV, and EBV plays an important role in the development of LEC. LEC showed positive squamous cell markers, indicating that the samples contain squamous cell carcinoma (SQCC). LEC is EBV (+) with nonkeratinizing SQCC, and this name better reflects the nature of this disease.
本研究旨在探讨淋巴上皮癌（LEC）的组织发生及其与爱泼斯坦-巴尔病毒（EBV）的关系。
采用原位杂交检测EBV的表达，并用免疫组织化学法检测45例LEC石蜡包埋组织中CK5/6、p63和p40的表达水平。
在来自10个不同样本的45例LEC石蜡包埋组织中，CK5/6阳性信号位于细胞膜。p63和p40的阳性信号位于细胞核。在所有LEC病例中，CK5/6、p63和p40的阳性率分别为93.3%（42/45）、95.6%（43/45）和93.3%（42/45）。EBV编码RNA（EBER）阳性信号位于细胞核。在45例LEC病例中，EBER表达呈强阳性，阳性率为100%（45/45）。
LEC与EBV密切相关，EBV在LEC的发生发展中起重要作用。LEC显示鳞状细胞标志物阳性，表明样本中含有鳞状细胞癌（SQCC）。LEC为EBV（+）的非角化SQCC，该命名更能反映本病本质。
