Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, P.R. China.
Department of Biochemistry, School of Life Sciences, Central South University, Changsha, Hunan, P.R. China.
J Cell Biochem. 2018 Jul;119(7):5222-5232. doi: 10.1002/jcb.26566. Epub 2018 Mar 12.
Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure, and large pore volume. Here we reported that the proliferation, differentiation, and mineralization were promoted in MC3T3-E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3-E1 cells cultured on MBG/T. Intriguingly, we observed that ERK phosphorylation was enhanced in MC3T3-E1 cells cultured on MBG/T. We showed that activated Runx2 in MC3T3-E1 cells cultured on MBG/T is through Erk1/2 phosphorylation. Consistent with this result, we also found that the expression of osteoblastic marker genes were increased. Therefore, we concluded that osteoblast differentiation and mineralization was enhanced after cells cultured on MBG/T through Erk1/2-activated Runx2 pathway. Our findings provided that MBG/T is a potential material in the process of bone repair.
介孔生物活性玻璃（MBG）是一种具有高骨传导性和骨诱导性的多孔材料，由于其具有高比表面积、有序的通道结构和大孔体积，在骨组织工程中具有广阔的应用前景。在这里，我们报道了吸附睾酮的 MBG（MBG/T）促进 MC3T3-E1 细胞的增殖、分化和矿化。我们发现，在 MBG/T 上培养的 MC3T3-E1 细胞中，关键转录因子 Runx2 的转录活性增加。有趣的是，我们观察到在 MBG/T 上培养的 MC3T3-E1 细胞中 ERK 磷酸化增强。我们表明，在 MBG/T 上培养的 MC3T3-E1 细胞中激活的 Runx2 是通过 Erk1/2 磷酸化实现的。与这一结果一致，我们还发现成骨细胞标记基因的表达增加。因此，我们得出结论，细胞在 MBG/T 上培养后，通过 Erk1/2 激活的 Runx2 途径增强了成骨细胞的分化和矿化。我们的研究结果表明，MBG/T 是骨修复过程中的一种潜在材料。
