Phillips S C
Department of Anatomy, Monash University, Clayton, Victoria, Australia.
Exp Neurol. 1989 Apr;104(1):68-72. doi: 10.1016/0014-4886(89)90010-1.
A nutritionally complete liquid diet containing 0, 3, 5, or 7% ethanol was fed to C57 mice for 4 months and killed after a further 4 months on a standard food pellet diet. The number of pyramidal cells in Araldite sections of the hippocampus was not significantly reduced in any group relative to the zero alcohol intake group. Synaptic counts in the area occupied by the pyramidal cell basal dendrites showed the 7% alcohol group to have a reduced number of synaptic contacts, with no convincing differences between the other groups relative to the zero alcohol group. All measurements made collectively could not be used to distinguish 3 or 5% ethanol consuming animals from 0% ethanol consuming animals. Significant differences in body weight and dry brain weight were detected in the comparison of the group treated with 7% ethanol and 4-month recovery with the group receiving the 0% ethanol dose and 4-month recovery. It is concluded that maintaining mice on a diet containing not more than 5% ethanol has no adverse long-term structural effects on hippocampal pyramidal cells or synapses.
给C57小鼠喂食含0%、3%、5%或7%乙醇的营养完全的流质饮食4个月，然后在换成标准食物颗粒饮食再饲养4个月后处死。相对于零酒精摄入量组，海马体环氧树脂切片中锥体细胞的数量在任何组中均未显著减少。在锥体细胞基底树突所占据区域的突触计数显示，7%酒精组的突触接触数量减少，相对于零酒精组，其他组之间没有明显差异。综合进行的所有测量都无法区分摄入3%或5%乙醇的动物与摄入0%乙醇的动物。在比较接受7%乙醇处理并恢复4个月的组与接受0%乙醇剂量并恢复4个月的组时，检测到体重和脑干重存在显著差异。得出的结论是，让小鼠食用含乙醇不超过5%的饮食对海马体锥体细胞或突触没有长期的不良结构影响。
